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《Acta Zoologica Sinica》 1991-04
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IMMUNOHISTOCHEMICAL LOCALIZATION OF VASOACTIVE INTESTINAL POLYPEPTIDE IN AMPHIOXUS

FANG YONG-QIANG~* HUANG WEI-QUAN~(**) QI XIANG~* and SU HUI-CI~(**) (Experimental Marine Biological Laboratory EMBL, Institute of Oceanology, Academia Sinica, Qingdao 266001)  
Wide distribution of vasoactive intestinal polypeptide (VIP) in amphioxus was found by immunohistochemical technique. The present study indicated that VIP immuno-positive endocrine cells existed in various regions of the digestive tract, for example, in mid-gut diverticulum, in anterior and posterior of mid-gut and in hind-gut. Many columnar endocrine cells in the front part of the mid-gut diverticulum showed strong immuno-reaction, but posteriormid-gut diverticulum was negative. Immuno-positive cells were found in the anterior region of mid-gut and the cytoplasmic top of these cells extended to the intestinal cavity, while other tissues were negative. A few scallered VIP endocrine cells in the posterior region of mid-gut were only found at the dorsum of the digestive tube. A lot of dense strong immuno-positive VIP endocrine cells were observed in the hind-gut. On the other hand, nerve cells and fibers in the brain vesicle and spinal cord showed immuno-positive reactions in different degrees. There were more than 10 nerve cells and fibers showing positive reaction at the dorsum of brain vesicle, the majority of them were nerve cells of small type, a few were large cells. The ventral region of the brain vesicle was negative. In addition, immunopositive reactions were also found in early spermatogenic cells, oogonia and subcutaneous tissues on both sides of the mouth cavity. These results supplied the morphological evidence for the physiological role of amphioxus and the evolution of polypeptide hormone.
【Fund】: 实验海洋生物学开放研究实验室资助项目 研究报告第26号。基金批文编号A20893006
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Chinese Journal Full-text Database 2 Hits
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【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 XU TIAN-LE, HUANG WEI-QUAN, SU HUI-CI (Department of Histology and Embryology, The Fourth Military Medical University, Xian 710032) FANG YONG-QIANG (Third Institute of Oceanography, SOA, Xiamen 361005);IMMUNOHISTOCHEMICAL LOCALIZATION OF CALMODULIN IN AMPHIOXUS[J];Acta Zoologica Sinica;1995-01
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