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《ACTA ZOOLOGICA SINICA》 1999-01
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RAPD ANALYSIS OF PIT-VIPERS OF THE GENUS AGKISTRODON IN CHINA

SHEN Xi ZHOU Kai Ya WANG Yi Quan (Biodiversity and Molecular Evolution Laboratory, Nanjing 210097, China)  
The phylogenetic relationship of pit vipers of the genus Agkistrodon from China was studied using RAPD technique. Totals of 33 samples of Agkistrodon and 2 samples of Vipera ursinii were used in this study, and phylogenetic relationships were inferred using UPGMA based on 72 RAPD makers which were amplified with 11 decamer primers. Each of the species clustered respectively first. Considerable intraspecific differentiation was found in A. intermedius. A certain genetic distance was detected among A. i. intermedius, A. i . saxatilis and Gansu samples and Ningxia samples of A. intermedius. A. shedaoensis showed a higher genetic distance to these subspecies (populations) of A. intermedius. The samples of A. brevicaudus from Jiangsu, Zhejiang and Anhui Provinces showed closer relationship among each other than that between them and the samples from Shaanxi Province. The samples of Agkistrodon from high altitude regions of both Gansu and Shaanxi Provinces probably should be referred to A. strauchi. A. ussuriensis is identified as the most basal lineage of the genus Agkistrodon from China. The results of RAPD analysis suggest that the genus Agkistrodon is a highly differentiated group.
【Fund】: 国家自然科学基金!资助项目 ( No.394 70 0 98)
【CateGory Index】: Q959.62
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