IMMUNOHISTOCHEMICAL STUDIES ON ENDOCRINE CELLS IN THE DIGESTIVE TRACT OF ALLIGATOR SINENSIS
WU Xiao-Bing; ZHANG Sheng-Zhou; CHEN Bi-Hui(Department of Biology,Anhui Normal University, Wuhu Z4100, China )DENG Ze-Pei; ZHOU Zhan-Xiang(College of Vetrinaty Medicine,Agricultural University of China, Brijing 100094 )WANG Chao-Lin; NIE Ji-Shan; XIE Wan-
The distribution and density of the endocrine cells in the digestive tract of Alligator sinensis were studied by immunohistochemical method with seven antisera of gut hormone. 5-Hydroxytryptamine (5-HT)-immunoreactive cells are distributed throughout the digestive tract with the highest density in the duodenum, the second highest in the oesophagus and rectum. Somatostatin-immunoreactive cells occured very numerously in the stomachus Pyloricus, moderately in the stomachus corpus, rarely in the stomachus cardiacus and occasionally in the duodenum. The gastrin-immunoreactive cells were located numerously in the duodenum and occasionally in the jejunum, ileum and rectum.The vasoactive intestinal polypeptide(VIP)-immunoreaetive cells were present mainly in the stomachus cardiacus, rarely in the stomachus corpus and pyloricus. Cells showing immunoreactivity to pancreatic polypeptide, glucagon and substance-P were not detected. Considering the feeding habit, the distributive mode of endocrine cells in the digestive tract of Alligator sinensis was discussed.