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《Acta Zoologica Sinica》 2001-04
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COMPARISON OF RAPD PATTERNS VARIATIONS IN 11 SPECIES OF CATANTOPIDAE

ZHENG Zhe-Min ① WANG Gui-Ling ② HUANG Yuan ① (①College of Life Science,Shaanxi Normal University, Xi'an\ 710062,Shaanxi,China) (②Fisheries College,Shanghai Fisheries Uinversity, Shanghai\ 200090,China)  
Catantopidae is a dominant family of Acridodea and has about over 3 000 speices. In order to investigate genetic relationships,random amplified polymorphic DNA(RAPD) technique was applied to assess RAPD patterns variations in 11 species belonging to 8 genera of Catantopidae. Among 23 arbitrary primers(10 bp)under predetermined optimal reaction conditions,8 primers were informative and yielded a total of 148 clear and reproducible bands. The patterns showed polymorphic variations among and within different genera and species. RAPD data were used to calculate Nei's similarity index. Molecular phylogentic tree were constructed by unweighted pair-group method with arithmetical averages (UPGMA). The results showed the species of each genus first clustered respectively and 8 genera of Catantopidae were divided into two branches, whose conclusion coincided with morpholgical (eg. wingmorphs). In one branch, 2 genera (Yunnanacris Chang and Pedopodisma Zheng)had short fore-wings, whereas 6 genera (Calliptamus Serville, Choroedocus Ⅰ. Bolivar, Shirakiacris Dirsh, Apalacris Walker, Xenocatantops Dirsh and Eucoptacra Ⅰ. Bolivar)in the other branch had long fore-wings. From these results, we found that the species with similar morphological characters shared a higher similarity on RAPD bands.
【Fund】: 国家自然科学基金资助项目 (No .395 70 110 )&&
【CateGory Index】: Q969
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