Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Zoologica Sinica》 2003-04
Add to Favorite Get Latest Update

Age-specific changes in androgen receptors in rat testis*

WANG Xiao-Yun ZHANG Jian LI Jian DUAN Xiang-Lin ** (College of Life Science,Hebei Normal University,Shijiazhuang 050016,China)  
In order to study age-specific changes in androgen receptors (ARs) we investigated changes in the expression of AR's in 60 SD rats, ranging in age from neonatal to 25 months. There were ten age groups: neonatal, 1 week, 2 weeks, 3 weeks, 1 month, 2 months, 3 months, 6 months, 12 months and 25 months. After rats were killed, their testes were immediately removed and fixed in Bouin's solution for up to 24 hours. Testes were dehydrated by a series of ethanol rinses, cleared in xylene and embeded in paraffin after which they were cut into 6 μm sections on a microtome. Immunocytochemistry analyses were performed after deparaffinage and rehydration. Primary antibodies were diluted 1∶100 in 0 01 mol/L PBS (pH 7 4). The control sections had the primary antibody omitted. Before incubation with the primary antibody, sections were pretreated in goat serum for 1 hour at 37℃. Following incubation with the primary antibody overnight at 4℃, sections were treated for 30 minutes in a biotinylated secondary antibody that matched the species of the primary antibody, followed by streptavidin/peroxidase complex incubation for 30 minutes at 37℃. Between incubation steps, the sections were rinsed three times for 15 minutes in PBS, but there was no PBS rinse between serum incubation and primary antibody incubation. Bound peroxidase was revealed by incubating the sections in 3, 3'-diaminobenzidine for 3-5 minutes. Sections were rinsed in PBS to stop the reaction, then dehydrated in a series of increasing concentrations of ethylalcohol. After being cleared in xylene, sections were coverslipped with a permanent mounting medium. We determined the average optical density of AR positive cells with an Image Analysis System. All data were analyzed using Excel and statistical significance determined with ANOVA. There were many kinds of AR-immunoreactive cells in normal rat testis, such as Leydig cells,endothelial cells,myoid cells,Sertoli cells, as well as spermatogenic cells. The distribution and staining intensity of AR-immunoreactive cells changed greatly with age. Our results show that: (1) There was a weakly positive expression of AR in Leydig cells in the first 3 weeks after birth. Positive expression of AR in Leydig cells peaked after 1 month but decreased markedly after 2 months after which it then increased gradually. (2) A weakly positive expression of AR in myoid cells was observed from in the first 2 weeks after birth. The positive expression of AR increased prominently in 3 week old rats but had decreased in 3 month old individuals,reaching its lowest level in 25 month old rats. (3) A strongly positive expression of AR in endothelial cells was observed in rats between 3 weeks and 2 months of age. It decreased prominently in 3 month old rats but there were no significant changes between 3 months and 25 months of age. (4) The positive expression of AR in Sertoli cells appeared at 1 month of age. In mature rats, the positive expression of AR in Sertoli cells changed with the spermatogenic cycle, but there was no expression of AR in Sertoli cells at 25 months. (5) The positive expression of AR in prospermatogonium cells appeared in neonatal rats. It appeared in spermatoons after 2 weeks, was observed in spermatogonia after 1 month and appeared in sperm after 2 months. But there was no expression of AR in spermatogenic cells at 25 months .
【Fund】: 河北省自然科学基金资助项目 (No. 3 0 0 164 )~~
【CateGory Index】: Q492
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 WANG Jian-li1,2, WANG Hui-chun1,3, TAI Fa-dao1,* (1. College of Life Sciences, Shaanxi Normal University, Xi’an 710062; 2. College of Life Sciences&Engineering, the North University for Ethnics, Yinchuan 750021; 3. Number One Middle Schoole in Linfen, Linfen 041000, China);Age Dependent Expressiones of Androgen Receptores in Testes and Epididymes of Mandarin Voles(Lasiopodomys mandarinus)[J];Zoological Research;2009-01
【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 WANG Li1,WU Xiao-bing1,XU Duo1,WANG Ren-ping2,WANG Chao-lin2(1.College of Life Science,Anhui Province Key Laboratory for Conservation and Exploitation of Biological Resource,Anhui Normal University,Wuhu 241000,China;2.Anhui Research Center for Chinese Alligator Reproduction,Xuanzhou 242034,China);Immunocytochemistry of Sex Steroid Receptors in the Testis of the Alligator Sinensis[J];Journal of Anhui Normal University(Natural Science);2008-06
2 YANG Xiao-zhen,CHEN Yao-xing,WANG Zi-xu,MA Yun-fei(College of Animal Medicine, China Agriculture University, Beijing, 100094,China);The Recent Progress of the Hormonal Regulation of Germ Cells Apoptosis in Male[J];Progress In Veterinary Medicine;2003-06
3 Li Kai, Cen Ying, Liu Xiaoxue, Luo Xusong. Department of Burn and Plastic Surgery, West China Hospital, Sichuan University, Chengdu 610041, China;The Effects of Nandrolone Phenylpropionate on Androgen Receptor of Liver and Sexual Glands in Burned Rats[J];Journal of Sichuan University (Medical Sciences Edition);2003-04
4 XU Xue - wen, YANG Dan - bo, CEN Ying, LUO Xu - song ( Department of Burned and Plastic Surgery, West China Hospital, Sichuan University, Chengdu 610041, China );Effects of Nandrolone phenylpropionate on testis and sex hormone in burned male rat[J];West China Journal of Pharmaceutical Sciences;2004-02
5 XIE Jin-xian1,WANG Nai-ping1,LI Ping1,LI Shang-qiu1,YUAN Juan-li2,LI Wei-neng1(1.Guangxi Traditional Chinese Medical University,Nanning 530001,Guangxi,China;2.Dept.of Pharmacology,Nanchang University Medical College,Nanchang 330006,Jiangxi,China);Effects of Osthole on Serum Testosterone and the Testis Androgen Receptor(AR) in the Reproduction System Disturbance Mice[J];Liaoning Journal of Traditional Chinese Medicine;2007-11
6 LIU Su-huan~(1,2),WANG Zhong-shan~(1)1.Department of Cell Biology,School of Basic Medical Science,Jilin University,Changchun,Jilin 130021,(China);2.Department of Physiology,Medical School of Qingdao University,Qingdao,Shandong 266021,China;Study on the Expression of Androgen Receptor in Testis,Epididymis and Prostate of Adult Rats with Diabetes[J];Nationlal Journal of Andrology;2005-12
7 Liu Suhuan, Zhao Hui, Xin Jili, Zou Donghui, Wang Zhongshan Dept. of Cell Biology, School of Basic Medical Sciences , Jilin University ( Changchun 130021) Liu Yijie Dept. of Radioactive Therapy, the General Hospital of the First Automobile Work;Changes of gene expression and the level of androgen receptor in reproductive glands of diabetic pubertal rat[J];Chinese Journal of Andrology;2002-05
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG BAY-CHAUR(Biology Department, Hwatung Pedagogical University);STUDIES ON THE ECOLOGY OF FOUR SPECIES OF LIZARDS IN HANGCHOWⅡ. BREEDING[J];Acta Zoologica Sinica;1966-02
2 WU MEIXI Fu FUYING(Department of Biology, Fujion Teachers University, Fuzhou);THE REPRODUCTIVE CYCLES AND BREEDING PATTERN OF THE SNAKE, ENHYDRIS CHINENSIS (GRAY)[J];Acta Zoologica Sinica;1989-01
3 JI Xiang SUN Ping Yue XU Xue Feng DU Wei Guo (Department of Biology,Hangzhou Normal College,Hangzhou 310036,China);RELATIONSHIPS AMONG BODY SIZE,CLUTCH SIZE, AND EGG SIZE IN FIVE SPECIES OF OVIPAROUS COLUBRID SNAKES FROM ZHOUSHAN ISLANDS,ZHEJIANG,CHINA[J];ACTA ZOOLOGICA SINICA;2000-02
4 FANG Yong-Qiang WENG You-Zhu HU Xiao-Xia (Third Institute of Oceanography, SOA, Xiamen 361005, Fujian, China);DISTRIBUTION OF SEX STEROID HORMONES AND THEIR RECEPTORS IN THE GONADS AND NERVOUS SYSTEM OF AMPHIOXUS (BRANCHIOSTOMA BELCHERI)[J];Acta Zoologica Sinica;2001-04
5 HU Jian-Rao 1,2 ,DU Ji-Zeng 1,JI Xiang 2** 1. College of Life Sciences, Zhejiang University, Hangzhou310027, Ch ina2. School of Life Sciences, Hangzhou Normal College, Hangzhou310036, Chin a;Annual variation in gonads of male Chinese skinks Eumeces chinensis[J];Acta Zoologica Sinica;2004-01
6 Liu Jun1,Zhong Fusheng1, Zhou Jiantao2,Zhou Xiaohai2(1.Hunan Environment-Biological Polytechnic,Hengyeng Hunan 421005;2.Yongzhou Peculiar Snake Company Ltd.Hunan);The Experiment of the Simulated Ecological Breeding the Zaocys dhumnades[J];Quarterly of Forest By-product and Speciality In China;2005-01
7 ZHENG Qiang Sun 1, ZHAO Zhan Yong 2, SHU Qing 3, LIU Jun 1, LI Jun Ling 3 1Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China, 2Department of Biology, Shaanxi Normal Llniversity,;Relationship between YNZ22 VNTR polymorphism and atherosclerosis suceptibility[J];Journal of The Fourth Military Medical University;2001-19
8 JIA Bin1, TANG Hong2, LI Wei3, CAI Wen-qing3 Faculty of Forensic Medicine1, Adult Education College2, Department of Urology, Second Affiliated Hospital3, Hebei Medical University, Shijiazhuang 050017, China;Effects of epidermal growth factor on cellular expression of estrogen and androgen receptors in mouse prostate[J];Journal of Southern Medical University;2006-10
9 Wu Ze, Ren Ping, Zhou Rong ,et al. The First Hospital of Yunnan Province, Kunming 650032;Quantitative Determine of Real-time Fluorescent PCR on the Expression of Leukemia Inhibitory Factor(LIF) in Early Pregnant Decidua[J];Chinese Journal of Family Planning;2005-02
10 LU Yu yan, LI Pi peng (Department of Biology,Yantai Normal College,Shandong Yantai 264025);Reproductive Characteristics of Rhabdophis tigrina Lateralis[J];JOURNAL OF ECONOMIC ANIMAL;1999-04
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 TANG Jin1,SHE Ruiping1,WANG Desheng2,WANG Yinhua1,YUE Zhuo1,XIA Kangkang1,TIAN Jijing1,DING Ye1,YIN Jun1,HAN Deping1 1.College of Veterinary Medicine,China Agricultural University,Beijing 100193,China 2.China Astronaut Research and Training Center,Beijing 100193,China;Effects of Simulated Weightlessness on Tissue Structure and AR and HSP70 Expressions in Testicle of Rat[J];Science & Technology Review;2008-19
2 Liao Hui1,Liu Jinghua1,Qian Huijun2,Lv Shengqi2 1.Department of Urology,Xiang fan Hospital Affiliated to Hubei College of TCM,Xiang fan 441000,China 2.Department of Urology,Renmin Hospital of Wuhan University;Effect of Sheng Jing He Ji mixture on pituitary-sexual gland axis[J];Chinese Journal of Andrology;2008-08
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved