Detecting the Y chromosome with C-banding and chromosome painting technique in the mandarin vole Microtus mandarinus
ZHU Bi-Cai ,GAO Jian-Guo ***,GAO Jun-Fang,ZHANG Yong,DONG Yu-Wei HOU Jin-Hui College of Life Science, Xuzhou Normal University, Xuzhou 221116,Jiangsu, China
The Y chromosome of Microtus mandarinus was detected with the C-banding technique and whole Y chromosome specific probe. Two C-bands on X chromosome were distinguished,a distinct one on the kinetochore, and a pale one on the short arm in the metaphase chromosome of the male Microtus mandarinus. We did not find the Y-chromosome which was dyed deeply. Metaphase chromosome and interphase nucleus of marrow cells of Microtus mandarinus were painted with the whole Y chromosome specific probe of mouse, and the metaphase chromosome and interphase nucleus of mouse marrow cells was used as the control. The painting results showed that the signal detection rate is about 0 to 2% in the metaphase chromosomes and 3% to 5% in the interphase nucleus of marrow cell of Microtus mandarinus, and the results are negative, but the control experiment was positive. From the results, we deduce that there are no homologic sequences of whole Y chromosome special DNA sequences of mouse in Y chromosome and whole genome of Microtus mandarinus. Perhaps, there are no important genes which determine male sex in the Y chromosome .