Effects of vitamin C polyphosphate on liver lipid peroxides and antioxidase activity in mice
ZHOU Xian Qing ** ,LI Sheng Li,WANG Xiao Hui, QIAO Xin Department of Laboratory Animal Sciences, Capital Medical University, Beijing 100054, China
The study was conducted to investigate the effects of vitamin C polyphosphate on liver lipid peroxides (LPO) and antioxidase activity in mice. Twenty four mice were fed with 35% vitamin C polyphosphate supplementation at the dosages of 0, 500, 2 500 , 5 000 mg/kg diets, respectively for 4 weeks. Livers were collected to analyze the content of LPO, and the activities of the superoxide dismutases (SOD), catalase (CAT) and glutathione peroxidase (GSH Px) . The LPO and SOD were determined with thiobarbituric acid reaction and nitrite form method, respectively; and the CAT and GSH Px with colorimetry method. The results showed that vitamin C polyphosphate had no significant effects on liver LPO levels in mice, although there was a decreasing tendency with an increase of vitamin C polyphosphate levels. The activities of SOD in the two groups supplemented vitamin C polyphosphate at 2 500 and 5 000 mg/kg diets were significantly higher than those of both control groups and the group supplemented vitamin C polyphosphate at 500 mg/kg. The activities of CAT in the two groups supplemented vitamin C polyphosphate at 2 500 and 5 000 mg/kg diets were higher than that of control group. The activity of GSH Px in the groups supplemented vitamin C polyphosphate at 5 000 mg/kg diets was notably higher than those of other three groups. These results suggest that vitamin C polyphosphate can improve the antioxidase activity, while the requirement of vitamin C polyphosphate for improving different antioxidase activity varies .