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《Acta Zoologica Sinica》 2005-03
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Expression of gene vasa during gonadal natural sex reversal of ricefield eel Monopterus albus

L Dao-Yuan 1, SONG Ping [DK3]1** , CHEN Yun-Gui 1, PENG Mao-Yu 1,GUI Jian-Fang 2 1. School of Life Sciences Wuhan University, Wuhan 430072, China 2. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China  
Using in situ hybridization with DIG antisense RNA probe, we detected the expression of gene vasa during gonadal development of Monopterus albus. During oogenesis, the expression of vasa mRNA can be detected in the cytoplasm of Ⅰ,Ⅱ, Ⅲ stage oocytes. The vasa mRNA concentration was not obvious in the cortical region of stage Ⅳ,Ⅴ oocytes. The signals of vasa mRNA can also be observed in degenerated oocytes. We detected some vasa mRNA positive cells in the membrane of Ⅲ,Ⅳ stage ovary, and these may be the primordial germ cells (PGC) which migrate to the membrane of the ovary and will differentiate to spermatogenia. These PGCs will migrate to the seminiferous tubule and develop into spermatids during sexual reversal. The expression of vasa mRNA in the mature testis is restricted to the spermatogenia and spermatocytes. We examined the Monopterus albus ovary and testis by alkaline phosphatase staining and found that there are alkaline phosphatase positive cells in the membrane of the ovary. In mature testis, the male germ cells in the gonadal sac are alkaline phosphatase positive while no alkaline phosphatase positive cells are found in the testis membrane. The results suggest that the male germ cells of Monopterus albus may derive from the PGCs in the membrane of ovary.
【Fund】: 国家“863”项目(2001AA222071);; 国家自然科学基金项目(30270675)资助~~
【CateGory Index】: Q786
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