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《Acta Zoologica Sinica》 2006-05
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The effect of high temperature on mammary epithelial cells proliferation and apoptosis

DU Juan1, DI He-Shuang1, GUO Liang2, LI Zhong-Hao1, CAI Ya-Fei3, WANG Gen-Lin 1**1. College of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095, China2. Department of Animal Science, Anhui Technology College, Fengyang 233100,Anhui,China3. College of Life Science, Anhui Normal University, Wuhu 241000,Anhui, China  
To study the biological and morphological characters of bovine mammary epithelial cells in response to heat stress, the cells were exposed to temperatures from 37 ℃to 41℃ for 1 hour, and the cell activities and morphological characters were measured by MTT, trypan blue dying, transmission microscopy, and flow cytometry. Results were shown as follows: (1) Cell proliferation was inhibited. The cell number in the treatment groups was significantly lower than that in the control(P0.05). During the recovery period, 6-18 hours at 37 ℃ after treatment,the cell numbers were lower and mitotic phases were shorter in heat-treated groups (treated for more than 5 days)than those in the control group(P0.05). (2)In response to heat stress, the mammary cell nuclei shrank and splintered,the organelles deformed and vacuolated, and the damaged cells developed into apoptosis bodies.(3)By flow cytometric analysis,the numbers of mammary cells arrested at G2 phase after heat shock was significantly higher than in controls(P0.01). It is concluded that heat stress inhibites mammary epithelial cell proliferation and induces cell apoptosis.
【Fund】: *国家科技部十五重大奶业专项( No.2002BA518A12);; 江苏省“十五”科技攻关项目( No.BG2003303);; 2005年度江苏省“研究生”创新计划项目 安徽省教育厅自然科学研究项目(No.2006KJ201B)~~
【CateGory Index】: S858.23;Q255
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【References】
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1 LI Zhong-hao1,KONG Li-juan1,LIU Qing-hua2,DU Juan1,WANG Li1,DI He-shuang1,WANG Gen-lin1(1.College of Animal Science and Technology,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China;2.Dairy Cattle Research Institute,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Relationship of antioxidant index in peripheral blood and lymphocyte apoptosis for Holstein dairy cattle under different temperature-humidity index[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2008-03
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1 Gao Yajun, Tang Shuang, Sun Sujun, Zhang YongInstitute of Bio-Engineering, Northwest A & F University, Yangling, Shanxi 712100, China;In Vitro Culture of Mammary Epithelial Cells in Goat[J];Anhui Agricultural Science Bulletin;2005-02
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