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《Acta Zoologica Sinica》 2007-01
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Isolation and characterization of the albinism-related genes of DEN-1 and DEN-2 from turbot Scophthalmus maximus

WANG Jia-Qing1,HOU Lin 1,ZOU Xiang-Yang2,YAO Feng1,LI Xiao-Yan11.College of Life Sciences,Liaoning Normal University,Dalian 116029,Liaoning,China2.Department of Biology,Dalian Medical University,Dalian 116027,Liaoning,China  
Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.Expression pattern analysis was performed by relative quantitative RT-PCR to analyze the expression levels of DEN-1 and DEN-2 in four kinds of skin tissues:ocular side of normal turbot(termed N1 skin),ocular side of albino turbot(termed A1 skin),blind side of normal turbot(termed N2 skin)and blind side of albino turbot(termed A2 skin).The optical densities of the signals of DEN-1 and DEN-2 normalized by β-actin gene show that DEN-1 and DEN-2 have the similar expression pattern:N1N2≈A2A1.A 497 bp sequence which had high homologies with the eya4 gene of many animals were produced by the in silico cloning.Electronic expression profile analysis of DEN-1 and DEN-2 in the different tissues,organs and development stages will provide valuable references for the further studies.
【Fund】: 辽宁省科技基金资助项目(No.2050819)~~
【CateGory Index】: S917.4
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