STUDIES ON GENETIC VARIATION AND PHYLOGENETIC RELATIONSHIPS AMONG SIX SPECIES OF PENAEUS GENUS BY RAPD MARKERS
SONG Lin Sheng XIANG Jian Hai LI Chen Xi ZHOU Lin Hua LIU Rui Yu (Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071,China) FAN Yong Jun (The Department of Biology, Nankai University,Tianjin 300071, China)
Random amplified polymorphic DNA (RAPD) technique was applied to assess the genetic variations among six species in the genus Penaeus. Amplifications with 20 primers under predetermined optimal reaction conditions gave 364 reproducible fragments ranging from 230 to 2 800bp. The amplified products were scored as present (1) or absent (0) for each DNA sample and an index of genetic similarity (F) was calculated. The value of (1 F) was used to evaluate genetic distances between species and a phylogenetic tree was constructed. From these results, we find that the species with similar morphological characters share a higher similarity on genomic DNA.Otherwise, the similarity is lower.