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《Northern Horticulture》 2007-05
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Application of Orthogonal Design in Rhododendron RAPD Reaction System

ZHANG Li,ZHOU Lan-ying,XIAO Qian-wen,WU Kai-zhi(College of Forestry and Horticulture,Sichuan Agriculture University,Yaan 625014)  
Essential factors affecting the result of Rhododendron RAPD-PCR were studied with orthogonal design.And the optimal RAPD-PCR were built as follow:80ng DNA template,0.4μmol/L Random primer,1.1mmol/LdNTPs,2.5mmol/LMgCl2and 0.9μmol/L in 20μL reaction solution.By analyzing the 6 factors and 5 levels,the results showed that the concentration of Mg2+,dNTPs and Random primer were the main factors,the concentration of Mg2+ had the greatest effect on the experiment.Low concentration of dNTPs and random primer can reach the requirement.
【CateGory Index】: S685.21
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