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《Northern Horticulture》 2008-01
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A Preliminary Analysis of Phylogenetic Relationships in Orchidaceae Based on cp rRNA ITS Sequence Data

BAI Rui1,HE Cong-fen1,DONG Yin-mao1,LI Lu-bin2,LIU Lei1,PENG Zhen-hua2(1.Beijing Key Lab of Plant Resources Research and Development,College of Chemistry and Environmental Engineering,Beijing Technology and Business University,Beijing 100037,China;2.Key Lab of Tree Breeding and Cultivation,State Forestry Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China)  
Noncoding sequences from the inverted repeat region of chloroplast DNA appear suitable for phylogenetic study at different taxonomic levels.In this study,we developed PCR primers against highly conserved regions of the rRNA operon located and used these to amplify the region spanning from 4.5 S rRNA gene to 5 S rRNA gene,which is roughly 450 bp.The sequence of cp rRNA ITS regions of 33 orchidaceous(12 Cymbidiums,14 Paphiopedilums,and 7 Dendrobiums)were analyzed using PCR amplification and direct DNA sequencing.The phylogenetic trees generated from maximum parsimony analysis showed that Paphiopedilum and Dendrobium are monophyletic group while Cymbidium is polyphyletic with C.lowianum which is sister to the remainder species,P.emersonii and P.malipoense became the sister group to the remainder species within Paphiopedilum.However,because of the low substitution rate in the rRNA ITS region,some of the clades identified received relatively low support,subgenus delimitations were also not clear.Further study is needed for achieving a robust phylogeny of Orchidaceae.
【Fund】: 国家林业局引进国际先进农业科学技术(948)项目(2004-4-26)及(2005-4-37)
【CateGory Index】: S682.31
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Chinese Journal Full-text Database 1 Hits
1 YANG Zhi-juan~(1,2),ZHU Gen-fa~(2*),ZHANG Xian~1(1 College of Horticulture,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China;2 Floricultural Research Institute of Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China);Research Advances of Orchidaceae Plants in Phylogeny and Genetic Relation[J];Acta Botanica Boreali-occidentalia Sinica;2005-09
Chinese Journal Full-text Database 10 Hits
1 XU Zhen-huan1,2 LIU Hong-Ke1,2 (1 The Sichuan academy of botanical engineering,Sichuan Zizhong 641200,China;2 Sichuan Yangchun Horticulture Co.LTD,Sichuan Chengdu 611730,China);Progress on synthetical of Cymbidium grandiflorium[J];Anhui Agricultural Science Bulletin;2008-19
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Chinese Journal Full-text Database 1 Hits
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