Full-Text Search：
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Northern Horticulture》 2008-04
Add to Favorite Get Latest Update

Comparison of Extraction Methods and ISSR Analysis of Genomic DNA in Japanese apricot(Prunus mume Sieb.et Zucc.)

GUI Teng-qin1,2,3,QIAO Ai-min3,SUN Min1,WANG Xin-yan3,SUN Xue-mei1(1.College of Life Science,Southwest University,Chongqing 400715,China;2.Dept.of Chemistry & Life Science,Qianxinan Teachers' College,Xingyi,Guizhou 562400,China;3.College of Agriculture and Landscape Architecture,ZhongKai University of Agriculture and Technology,Guangzhou,Guangdong 510225,China)  
Genomic DNA of Japanese apricot(Prunus mume Sieb.et Zucc.) was extracted from the leaves of three cultivars of Japanese apricot,'yuanyangmei''huanghoumei' and 'xiaoshanxuan'.Considering that Japanese apricot is rich in secondary metabolites such polyphenols,saccharide and terpene and so on.Three methods were adopted to isolate genomic DNAs of Japanese apricot.The results show that the modified CTAB method is more effective,the quality and quantity of genomic DNA extracted can used at all kinds studied of molecular biology for Japanese apricot.OD260/280 was 1.80 or so.When tested through the DNA-ISSR analysis,experiment requirement was met perfectly.In addition,the key steps and main characters in the modified CTAB method were analyzed in detail.
【Key Words】： Japanese apricot(Prunus mume Sieb.et Zucc.) Genomic DNA DNA extraction CTAB SDS
【Fund】： 广东省自然科学基金资助项目(B1010412)
【CateGory Index】： S662.4
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 ZHAO Jing,YE Huan,LI Xue-song,LI Jian-hua(College of Life Science and Technology,Xiaogan University,Xiaogan,Hubei 432003);Comparative Research of Four Kinds of Modified CTAB Extraction of Genomic DNA of Celtis koraiensis Nakai[J];Northern Horticulture;2010-01
【Citations】
Chinese Journal Full-text Database 5 Hits
1 Ming Jun;Zhang Qixiang;Yan Xiaolan;Qiu Lijuan. Department of Horticulture, Hubei Agricultural College, Jingzhou, 434025, P. R. China.;DNA template preparation and technology system building for AFLP analysis of Prunus mume.[J];Journal of Beijing Forestry University;2003-03
2 Zhao Changling;Guo Weiming;Chen Junyu. College of Life Sciences, Nanjing Agricultural University, Nanjing,210095,P. R. China.;Preliminary study on the categories and contents of the flower color pigments of Prunus mume Sieb. et Zucc[J];Journal of Beijing Forestry University;2004-02
3 Yang Jian1, Tang Xuwei1, Tu Bingkun2 ,and Xie Puqing3(1Department of Biological Applied Engineering, Shenzhen Polytechnic, Shenzhen,Guangdong 518055 China; 2Department of Forestry, Huazhong Agricultural University, Wuhan, Hubei 430070 China; 3Wuhan Municipal Institute of Forestry and Pomology, Wuhan, Hubei 430075 China);RAPD Analysis of Three Chinese Endemic Castanea Species[J];Journal of Fruit Science;2004-03
4 Xu Dechang; Zhao Yahua; Dutiankui (Ningxia Agricultural College, Yongning, Ningxia, 750105);STUDY ON THE EXTRACTION AND PURITY OF PLANT TOTAL DNA AND NUCLEAR DNA[J];JOURNAL OF NINGXIA AGRICULTURAL COLLEGE;1997-03
5 SHEN Yong-bao.SHI Ji-sen (Southern Forest Tree Seed Center of National Forestry Bureau, Nanjing 210037,China);DNA Extraction from Different Tissues of Ginkgo and Chinese Chestnut[J];Journal of Nanjing Forestry Unversity;2001-06
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 FANG Cong bing 1,SHENG Bin cheng 2,ZHANG Zhen 2 (1 Department of Horticulture,Anhui Agricultural University,Hefei 230036; 2 Department of Horticulture,Nanjing Agricultural University);Effect of Different Sample-making Methods for SEM on Pollen Morphology of Fruit Mei (Prunus mume Sieb et Zucc)[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2000-01
2 Chen Junyu;A NEW SYSTEM OF CLASSIFICATION FOR CHINESE MEI FLOWER (PRUNUS MUME SIEB.ET ZUCC.) CULTIVARS[J];;1981-02
3 Zhang Qixiang;A COMPARATIVE STUDY ON DIFFERENCES IN COLD HARDINESS IN SOME OF MEI FLOWER CULTIYARS (PRUNUS MUME SIEB. ET. ZUCC)[J];;1985-02
4 Zhang Qixiang;OBSERVATION OF POLLEN GERMINATION OF MEI FLOWER INTERSPECIFIC POLLINATION AND THEIR PARENTS UNDER THE CONDITION OF 0—2℃[J];Journal of Beijing Forestry University;1986-01
5 Zhang QiXiang;THE INTERSPECIFIC CROSSING OF MEI FLOWER AND COLD HARDINESS BREEDING[J];Journal of Beijing Forestry University;1987-01
6 Zhang Qixiang (Dept. of Landscape Horticulture);The Interspecific Cross Experiments and Breeding for Hardiness in Mei Hua (Prunus mume Sieb. et Zucc.) (Ⅱ) Studies on Freezing-Resistance of the Interspecific Hybrids and Their Parents[J];Journal of Beijing Forestry University;1988-04
7 Mao Hanshu Ma Yan Wang Zhongzhi(Beijing Forestry University);Studies on Numerical Classification of Chinese Mei Flower Cultivars[J];Journal of Beijing Forestry University;1992-04
8 Chen Jun-yu.College of Landscape Architecture, Beijing For. Univ., 100083, P.R.China.;Sixty years in researching Mei flowers.[J];Journal of Beijing Forestry University;2002-Z1
9 MING Jun; ZHANG Qixiang. College of Horticulture and Landscape Architecture, Yangtze University, Jingzhou, 434025, P. R. China.;AFLP DNA fingerprinting analysis of close genetic relationships of cultivars of Prunus mume Sieb. et Zucc[J];Journal of Beijing Forestry University;2004-05
10 Li Qingwei (College of Landscape Architecture, Beijing Forestry University,100083, P. R. China);Features of Planning and Construction of International Mei Garden in Beijing[J];Journal of Beijing Forestry University;2004-S1
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Chen Jun-yu.College of Landscape Architecture, Beijing For. Univ., 100083, P.R.China.;Sixty years in researching Mei flowers.[J];Journal of Beijing Forestry University;2002-Z1
2 Liu Qinglin (Department of Ornamental Horticulture, China Agricultural University, Beijing, 100094, P.R. China) Chen Junyu (College of Landscape Architecture, Beijing For. Univ., 100083, P.R. China);A Preliminary Research on the Relationship of Prunus mume Cultivars and Its Close Relatives by Using RAPD Assay[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-02
3 PENG Jian ying 1, SHU Huai rui 2, PENG Shi qi 1 (1 Research Center of Chinese Jujube, Agricultural University of Hebei, Baoding 071001, China; 2 Department of Horticulture, Shandong Agricultural University, Tai′an 271018, China);A method for genomic DNA isolation of Chinese jujube and acid jujube[J];Journal of Agricultural University of Hebei;2000-04
4 YANG Jian 1, TU Bing-kun 2, XIE Pu-qing 1, WANG Ai-xin 1 [WT8.5BZ]( 1.Wuhan Municipal Institute of Forest and Pomology, Wuhan 430075, China; 2.The Department of Forest,Huazhong Agricultral University , Wuhan 430070, China ) [WT9.5HZ];The main varieties of Castanea chestnut and its wild relative species in Hubei province[J];Guihaia;2001-02
5 Zhang Qinying Chen Jun-Yu (College of Landscape Architecture,Beijing For.Univ.,100083,P.R.China);Research Advances in Prunus mume[J];Journal of Beijing Forestry University;2003-S2
6 Zhao Changling Guo Weiming (College of Horticulture,Nanjing Agricultural University,210095,Nanjing);Mei Flower and Tree Peony:the First Choice of the National Flower of China[J];Journal of Beijing Forestry University;2003-S2
7 Yi Ganjun; Huo Heqiang; Cai Changhe; Qiu Yanping; Huang Ziran; Chen Dacheng(1 Pomology Research Institute, Guangdong Academy of Agric. Sci., Guangzhou, 510640;2 Dept. of Sericulture, South China Agric. Univ.;3 Dept. of Horticulture, South China Agric. Uni;DNA Isolation Procedure for AFLP Amplification Reaction of Litchi[J];JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY;1999-03
8 MENG Xiang-chun, ZHANG Yu-jin, WANG Xiao-qing (Department of Biology, South China Normal University, Guangdong Key Lab of Technology for Plant Development, Guangzhou 510631, China);CONTENT CHANGES OF ANTHOCYANIN, REDUCING SUGAR AND SOLUTABLE PROTEIN DURING THE FLOWER DEVELOPMENT OF PETUNIA HYBRIDA[J];Journal of South China Normal University(Natural Science);2001-02
9 WANG Yan 1 LI Shaoshan 2 LIU Songhao 2 (1 School of Life Science and Technology, Jinan University , Guangzhou 510632) (2.Institute of Laser Life Science, South China Normal University, Guangzhou 510631);Quick Preparation of Total DNA from Plants[J];ACTA LASER BIOLOGY SINICA;2000-01
10 Yin TongmingHan ZhengminHuang MinrenWang MingxiuLi Shuxian (Forest Genetics and Gene Engineering Laboratory Nanjing Forestry UniversityNanjing210037);RAPD Experiment Protocol and Optimization in Forest Trees[J];JOURNAL OF NANJING FORESTRY UNVERSITY;1999-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved