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Effects of Sucrose,Boron and Calcium on in Vitro Pollen Germination and Pollen Tube Growth of Prunus triloba Lindl

DU Yu-hu,QI Ying,JIANG Jin-biao,YANG Chun-ling,ZHENG Shuang,LI Yan(Liaoning Agricultural Vocation-Technical College,Yingkou,Liaoning 115009,China)  
The effects of the different concentrations of sucrose,calcium and boron on pollen germination and pollen tube growth of Prunus triloba var.plena Dipp.and Prunus triloba var.simplex were investigated.The results showed that 5%~10% of sucrose was not conducive to pollen germination and pollen tube growth,while the concentrations at 15%~30% could improve.The optimum concentration of sucrose was 25%,both average pollen germination and pollen tube length for 38.72% and 489.6 μm.0.01% to 0.07% of exogenous calcium can significantly promote the pollen germination and pollen tube growth of Prunus triloba in vitro,0.03% of the largest.0.01% of boron on Prunus triloba var.simplex and 0.01% to 0.03% of boron on Prunus triloba var.plena Dipp.pollen germination and pollen tube growth of a significant role in promoting,suggested that the Prunus triloba var.plena Dipp.more needs boron than Prunus triloba var.simplex.
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