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《Northern Horticulture》 2008-08
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Rapid Propagation in Vitro of Stem-segment with Single Bud of Chrysanthemum

YAN Xiang-ping(The Research Institute of Vegetables,Xining,Qinghai 810016,China)  
Withminimal media MS axillary innovations of Chrysanthemum recommended variety 'Wansheng' were used as explants to be cultured in vitro.The results showed that MS+BA 2.0~3.0mg/L+NAA 0.1mg/Lwas the appropriate medium to induce buds,MS+BA 0.5mg/L+NAA 0.1mg/L was the most effective medium for subculture,and 1/2 MS+IBA 0.15mg/L was ideal medium to root.Hardening seedlings in perlite,the survival rate could be 100%.The similar result was obtained after transplanting tube seedlings to nutritive soil directiy with survival rate being 95.6%.The latter method was proved easily and effectually.
【CateGory Index】: S682.11
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