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《Northern Horticulture》 2009-04
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Pollen in vitro Culture and Viability Detection of Self-pollen on Radish

LIU Chun-xiang,LIU Ming,ZHAO Guang-qiang,CHEN Ai-jun(Bio-Engineering Department of Weifang University,Weifang,Shandong 261061,China)  
The two optimum pollen solid culture medium of radish were selected from 18 medium,under the condition of relative humidity above 95%,at 25℃,4 hours growing time.One was sucrose 10%,agar 0.5%,and boric acid 0.02%,the other was sucrose 5%,agar 0.3%,and boric acid 0.04%,the pollen germination rate were more than 97% under these conditions.This study analyzed pollen fertility by combining self-pollination with observing the tube growth state in the stylous under microscope and under UV light by 0.1% aniline blue to tests the viability of the radish pollen by staining.Chemical staining method was tried to detect pollen viability,too.The result showed that 0.1% aniline blue staining can detect whether self-pollen tube can pass through their chapiter for self-incompatibility analysis,and potassium iodide was not fit for radish pollen viability detection.
【Fund】: 国家星火计划资助项目(2007EA740101)
【CateGory Index】: S631.1
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