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《Northern Horticulture》 2010-03
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Research Progress of IPCR on Cioneing Flanking Sequence

WANG Jing (Inner Mongolia Key Laboratory of Herbage and Endemic Crop Biotechnology,Biotechnology Center,College of Life Sciences,Inner Mongolia University,Hohhot 010021)  
IPCR is the first flanking cloning technique,which relied on PCR Using one reaction,IPCR process could clone two flanking ends of the known sequence.Combined with the technique of LD-PCR,RACE and SMART,it is widely used.However,in IPCR process,there are some problems such as the insensitivity of the reaction system and some unknown reasons which influenced the reaction process.This report summarized the achievement and the development of the IPCR during the past two decades,and proposed the improvement reaction system in order to give some inspirations for the future.
【CateGory Index】: Q503
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 Hong Dengfeng Wan Lili Yang Guangsheng*National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement (Wuhan Branch), Wuhan, 430070;Estimation of Techniques about Cloning the Flanking DNA Sequence[J];Molecular Plant Breeding;2006-02
2 HONG Yu-zhi,XIAO Ya-zhong~*,FANG Wei,TONG Pin-gui,YUAN Jing,SUN Qin-ying(School of Life Science,Anhui University,Anhui Key Laboratory of Eco-engineering and Bio-technique,Hefei 230039,Anhui);Long Distance-inverse PCR Technique for Efficient Cloning of Flanking Sequences Adjacent to Known DNA Fragments[J];Acta Laser Biology Sinica;2006-01
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