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《Northern Horticulture》 2010-13
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Establishment of AFLP Analysis System and its Application in Chaenomeles

YIN Chang-hong,MA Yan,ZANG De-kui (Forestry College,Shandong Agricultural University,Tai'an,Shandong 271018)  
Twenty-nine cultivars in the genus Chaenomeles were used as materials to establish the AFLP(Amplified Fragment Length Polymorphism)reaction system for studying the relationship and genetic diversity in flowering quince resources.The AFLP analysis system was established by detected the results of several reactions such as DNA extraction,enzymes restriction,pre-amplification and selective amplification;the main factors which affected AFLP analysis were analyzed.Eight pairs of primers,E-AAG+M-CAA,E-ACA+M-CAA,E-AAG+M-CAC,E-AAG+M-CAG,E-AAG+M-CAT,E-AAG+M-CTG,E-ACA+M-CTT,and E-ACG+M-CTT,were selected to selectively amplify the genomic DNA of the cultivars,and clear amplification bands were obtained.The results suggested that the reaction system was good for Chaenomeles AFLP analysis.
【Key Words】： Chaenomeles AFLP extraction of DNA primer combinations
【Fund】： 国家自然科学基金资助项目(30972406);; 山东省优秀中青年科学家奖励基金资助项目(2004BS06003)
【CateGory Index】： S661.6
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【References】
Chinese Journal Full-text Database 1 Hits
1 WANG Kai-Yu;MOU Qiao-Feng;HUANG Jin-Lu;CHEN De-Fang;HUANG Ling-Yuan;WANG Jun;LIAN Hai;Fisheries Department of Sichuan Agricultural University;Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University;;AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF AEROMONAS VERONII[J];Acta Hydrobiologica Sinica;2013-05
【Citations】
Chinese Journal Full-text Database 10 Hits
1 Ming Jun;Zhang Qixiang;Yan Xiaolan;Qiu Lijuan. Department of Horticulture, Hubei Agricultural College, Jingzhou, 434025, P. R. China.;DNA template preparation and technology system building for AFLP analysis of Prunus mume.[J];Journal of Beijing Forestry University;2003-03
2 Wang Caihong1， Wang Qian2， Dai Hongyi3， Jia Jianhang2 ， Wang Bin2，and Shu Huairui1 （1Department of Horticultural Science， Shandong Agricultural University， Taian 271018 2Genetic Institute of Chinese Academy of Science， Beijing 10;Cloning an AFLP Marker of Columnar Gene（Co） of Apple[J];Journal of Fruit Science;2001-04
3 Lu Guangyuan\ Yang Guangsheng\ Fu Tingdong (Huazhong Agricultural University, Wuhan 430070);Silver-Stained Aflp- A Novel Assay for DNA Fingerprinting in Brassica napus[J];Journal of Huazhong Agricultural;2001-05
4 CHEN Qi-liang [JP4](Institute of Fruit Tea Silkworm & Mulberry,Hubei Academy of Agricultural Sciences,Wuhan,Hubei 430209,China)LI Qing-guo (Wuhan Dongxihu Institute of Horticulture Sciences,Wuhan,Hubei 430040,China)TIAN Rui (Institute of Fruit Tea Silkworm & Mulberry,Hubei Academy of Agricultural Sciences,Wuhan,Hubei 430209,China)ZHANG Qing-lin (College of Horticulture and Forestry,Huazhong Agricultural University,Wuhan,Hubei 430070,China);AFLP Technique and Its Application in the Studies of Horticultural Plants’ Genetics and Breeding[J];Journal of Yangtze University(Natural Science Edition);2005-02
5 WANG Xian~1,CHAI Yong-sheng~2,ZHI Yong-pu~3 (1.College of Forestry and Horticulture,Henan Agricultural University, Zhengzhou 450002, China; 2.College of Natural Resource and Environment,Beijing Forestry Univerisity, Beijing 100083, China; 3.Land Resource Bureau of Huojia County. Huojia 453800, China);Extraction of DNA and construction of AFLP fingerprinting in leaves of Lagerstromi indica[J];Journal of Henan Agricultural University;2004-02
6 ZHANG Chun-qing 1,YANG Kai 2,JIA Ji-zeng 2 (1.Agronomy College,Shandong Agricultural University,Taian 271018,China; 2.Crop Germplasm Institute,Chinese Agricultural Academy,Beijing,100081);DNA FINGERPRINT TECHNIQUE--AFLP OPTIMIZATION[J];Journal of Shandong Agricultural University;2002-01
7 GOU Ben-fu~(1,2),XIE Ying~3,XIONG Yun-hai~2,ZOU Guo-lin~1(1.School of Life Science,Western Chongqing College,Chongqing 402168,China;2.College of Life Science,Wuhan University,Wuhan 430072,China;3.Hospitcal of Traditional Chinese Medicine of Yongchuan City,Chongqing 402100,China);Preliminary study on genetic diversity of Rosa davunica by AFLP[J];Southwest China Journal of Agricultural Sciences;2004-03
8 Zhang Junxiang~1, Li Zhilin~2, Fan Chengming~1, Cheng Shaoli~1, Zhao Mingfu~1, and He Yueqiu~ 1* ( ~1College of Plant Protection, Yunnan Agricultural University,Kunming, Yunnan 650201, China; ~2College of Gardening and Horticulture, Yunnan Agricultural University, Kunming,Yunnan 650201, China);Phylogenetics Analysis of the Chinese Orchids in Yunnan Province Using AFLP Technique[J];Acta Horticulturae Sinica;2006-05
9 Ming Jun 1* and Gu Wanchun 2(1Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China);Genetic Diversity in Natural Populations of Syringa oblata Detected by AFLP Markers[J];Acta Horticulturae Sinica;2006-06
10 Liu Ping,Wang Zicheng,and Shang Fude*(College of Life Science,Agricultural Biotechnology Institute,Henan University,Kaifeng,Henan 475001,China);AFLP Analysis of Genetic Diversity of Paeonia suffruticosa Cultivars in Henan Province[J];Acta Horticulturae Sinica;2006-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 DU Chuan-yin1,3,4,WANG Yu-jun2,LI Si-shen3,LI Chang-bao3,LIU Hong-xiang4,TIAN Ji-chun3 (1.Weifang Tobacco Limited Corporation,Weifang 261031,China;2.Plant Protection College,Shandong Agricultural University,Taian 271018,China;3.Agronomy College,Shandong Agricultural University,Taian 271018,China;4.Qingzhou Tobacco Research Institute of CNTC,Qingdao 266061,China);Construction of AFLP Silver-staining Analysis System in Tobacco[J];Shandong Agricultural Sciences;2007-05
2 ZHANG Shu1,2,LI Li-bin2,WANG Feng-de2,LIU Li-feng2,LI Hua-yin2,GAO Jian-wei1,2 GAO Ling1(1.College of Life Science,Qingdao Agricultural University,Qingdao 266109,China;2.Vegetable Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China);Analysis of Differentially Expressed Genes in Brassica rapa L.ssp. pekinensis,Raphanus sativus L. and Their Hybrid Using cDNA-AFLP[J];Shandong Agricultural Sciences;2011-07
3 YANG Dong-zhi et al(Department of Biology,Zhengzhou University,Zhengzhou,Henan 450001);Study on Cultivar Classification of Chinese Cymbidiums[J];Journal of Anhui Agricultural Sciences;2007-29
4 GOU Ben-fu(College of Life Science,Chongqing University of Arts and Sciences,Chongqing 402160);AFLP Analysis of Genetic Polymorphism of Five Lines Rose[J];Journal of Anhui Agricultural Sciences;2008-17
5 WENG Qiao-yun et al(College of Agriculture and Forestry Science and Technology,Hebei North University,Zhangjiakou,Hebei 075000);Selection of AFLP Markers Linked to Setaria italica Beauv.Resistance to Leaf Rust[J];Journal of Anhui Agricultural Sciences;2012-02
6 HUANG You-kai et al(College of Forestry and Landscape,Anhui Agricultural University,Heifei,Anhui 230036);Research Advances in the Application of Molecular Markers in the Genetic Breeding of Orchid[J];Journal of Anhui Agricultural Sciences;2012-12
7 HAN Li-xiao et al(China National Engineering Research Centre for Floriculture,College of Landscape Architecture,Beijing Forestry University,Beijing 100083);Research Progress of Molecular Marker in Tree Peony[J];Journal of Anhui Agricultural Sciences;2012-16
8 ZHU Hong-xia(Shanghai Polytechnic College of Urban Management,Shanghai 200438 China);DNA Template Preparation and Technology System Construction for AFLP Analysis in Paeonia suffruticosa[J];Northern Horticulture;2007-10
9 GUI Teng-qin1,2,3,QIAO Ai-min3,SUN Min1,WANG Xin-yan3,SUN Xue-mei1(1.College of Life Science,Southwest University,Chongqing 400715,China;2.Dept.of Chemistry & Life Science,Qianxinan Teachers' College,Xingyi,Guizhou 562400,China;3.College of Agriculture and Landscape Architecture,ZhongKai University of Agriculture and Technology,Guangzhou,Guangdong 510225,China);Comparison of Extraction Methods and ISSR Analysis of Genomic DNA in Japanese apricot(Prunus mume Sieb.et Zucc.)[J];Northern Horticulture;2008-04
10 ZHAO Zhi-chang1,HU Gui-bing2,LIU Yun-chun2,OU Yang-ruo2,LUO Shi-rong3(1.Key Laboratory of Bio-resources and Eco-environment of Ministry of Education,School of Life Science,Sichuan University,Chengdu,Sichuan 610064,China;2.College of HorticuLtural Science,South China Agricultural University,Guangzhou,Guangdong 510642,China;3.Tropical Germplasm Resourse Research Institute,CATAS,Danzhou,Hainan 571737,China);DNA Extraction from Sweetsop (Annona squamosa L.) and Establishment of AFLP Analysis System[J];Northern Horticulture;2009-10
China Proceedings of conference Full-text Database 6 Hits
1 Li Xinhai Yuan Lixing Li Mingshun (Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Beijing 100081);Methods and Techniques of Variety Purity Test in Maize[A];[C];2001
2 Wang Qiang, Zhang Maojun ( Pomology Research Institute Jilin Academy of Agricultural Sciences,Gongzhuling Jilin 136100, China);Application of Molecular Marker Technology to Heredity and Breeding of Malus Mill.[A];[C];2004
3 SHEN Peng FENG Yu-lan DONG Li (College of Landscape Architecture,Beijing Forestry University, National Engineering Research Center for Floriculture,Beijing 100083);Investigation on Resources of Hemerocallis middendorfii in North-east of China[A];[C];2009
4 ;Genetic Diversity in the Natural Populations of Primula cockburniana Revealed by AFLP Molecular Markers[A];[C];2011
5 Tian Zhendong Xie Conghua Liu Jun Hu Cheng (Huazhong Agricultural University,Hubei Wuhan 430070);STATUS AND PROSPECTIVE OF MOLECULAR BREEDING OF POTATO LATE BLIGHT RESISTANCE Late Blight Resistance[A];[C];2001
6 HU Cheng XIE Cong-hua TIAN Zhen-dong LIU Jun (Huazhong Agricultural University,WUHAN 430070);ADVANCES OF MOLECULAR BIOLOGY ON POTATO DISEASE RESISTANCE[A];[C];2002
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 QIN Guo-min et al (Key Laboratory of Oceanic Biotechnology of Jiangsu Province,Huaihai College of Technology,Lianyungang,Jiangsu 222005);Infection of Aeromonas veronii from Diseased Cyprinus carpio L.and the Biological Characteristics of Its Pathogen[J];Journal of Anhui Agricultural Sciences;2008-19
2 HU Wang et al(Fisheries Research Institute,Anhui Academy of Agricultural Sciences and Fisheries,Hefei,Anhui 230031);Study on the Separation,Isolation and ERIC-PCR Fingerprinting of Aeromonas hydrophila from Parabramis pekinensis with Hemorrhagic Disease[J];Journal of Anhui Agricultural Sciences;2012-04
3 YANG Bao-ming,LV De-guo,QIN Si-jun,HUANG Zuo-gang(College of Horticulture,Shenyang Agricultural University,Shenyang 110161);Effects of Waterlogging on the Activities of Protective Enzyme System and Photosythesis of Hanfu'Apple[J];Northern Horticulture;2007-08
4 LIN Qi-cun,CAI Li-juan,YANG Zhong-jing,YU Xia-kui,FENG Xiao-yu(Hangzhou Institute of Agricultural Science,Hangzhou 310024,China);Isolation,Identification and Artificial Infection Test of Pathogen from Ulcerous Tinca tinca[J];Fisheries Science;2005-11
5 Fang Yifu, Wu Xiaoxing, Li Changgui(Shandong Provincial Academy of Forestry Science, Ji’nan 250014, P. R. China); Tian Ming(Shandong Agricultural University); Wang Cuixiang, Hu Dingmeng(Shandong Provincial Academy of Forestry Science);Cold Resistance of Eleven Evergreen Broad-leaved Species by Electrical Conductivity Method[J];Journal of Northeast Forestry University;2007-12
6 LI Shan, ZHAO Guifang(Biodiversity research center of Mts. Qinling, Northwest University, Xi'an,710069,China);AFLP molecular marker and its application[J];Acta Botanica Boreali-occidentalia Sinica;2003-05
7 CEN Xian-chao1,2,PENG Fang-ren1(1.College of Forest Resources and Environment Nanjing Forestry University,Nanjing 210037,Jiangsu,China;2.Forestry Bureau of Qiannan Autonomous Prefecture,Duyun 558000,Guizhou,China);The Chlorophyll Fluorescence Characteristics of Different Varieties (Types) of Catalpa bungei under Water Stress Condition[J];Journal of Fujian Forestry Science and Technology;2010-02
8 Lü Hai-Tao,Sun Hai-Feng,Qu Bao-Han,Dai Hong-Yi (Qingdao Agricultural University,Qingdao 266109);Simultaneous Determination of Six Phenolic Compounds in Apple Juice by High Performance Liquid Chromatography[J];Chinese Journal of Analytical Chemistry;2007-10
9 Mamut Kurban,Mahrra Mamut and Kamlbik Umar(College of Chemistry and Chemical Engineering,Xinjiang University,Urumqi 830046);Determination of total saponin from cydonia ablonga mill in Xinjiang by spectrophotometry[J];Chinese Journal of Analysis Laboratory;2008-02
10 Wang ZhonghuaInstitute of Biotechnology, Zhejiang Wanli University, Ningbo, 315100;DNA Fingerprinting Technology and its Application in Crop Germplasm Resources[J];Molecular Plant Breeding;2006-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Zhang Qixiang(Department of Landscape Architecture, BFU);Studies on Cultivars of Crape-Myrtle(Lagerstroemia indica)and Their Uses in Urban Greening[J];Journal of Beijing Forestry University;1991-04
2 Ming Jun;Zhang Qixiang;Yan Xiaolan;Qiu Lijuan. Department of Horticulture, Hubei Agricultural College, Jingzhou, 434025, P. R. China.;DNA template preparation and technology system building for AFLP analysis of Prunus mume.[J];Journal of Beijing Forestry University;2003-03
3 Wu Guoliang Yang Zhihong Liu Qunlong (Department of Horti.,Shanxi Agri.Univ.,Taigu,030801,P.R.China);The Biological Characteristics of Flowering and Pollination in Early Lilac and Its White Flowered Variety[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1998-02
4 Liu Qinglin (Department of Ornamental Horticulture, China Agricultural University, Beijing, 100094, P.R. China) Chen Junyu (College of Landscape Architecture, Beijing For. Univ., 100083, P.R. China);A Preliminary Research on the Relationship of Prunus mume Cultivars and Its Close Relatives by Using RAPD Assay[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-02
5 Leng Pingsheng Yang Xiaohong Hu Yue Zhu Huiying (Beijing Agricultural College,Beijing102206);Studies on the Characteristics of Photosynthesis and Transpiration of Five Gardening Trees[J];Journal of Beijing Agricultural College;2000-04
6 ZHANG Gui xia,CHEN Jing,WANG Wen jiang,MA Xue dong(Mountainous Areas Research Institute, Agricultural University of Hebei, Baoding 071001, China);Amplified fragment length polymorphism(AFLP) molecular marker technique and its application in the studies of fruit trees[J];Journal of Agricultural University of Hebei;2003-S1
7 Fang Jinggui Liu Dajun Ma Zhengqiang * Key Lab for Crop Genetic and Germplasm Innovation of China, Nanjing Agricultural University, Nanjing, 210095;Constructing Mango (Mangifera indica L.) Genetic Map Using Markers for Double Heterozygous Loci[J];Molecular Plant Breeding;2003-03
8 Wang Caihong1， Wang Qian2， Dai Hongyi3， Jia Jianhang2 ， Wang Bin2，and Shu Huairui1 （1Department of Horticultural Science， Shandong Agricultural University， Taian 271018 2Genetic Institute of Chinese Academy of Science， Beijing 10;Cloning an AFLP Marker of Columnar Gene（Co） of Apple[J];Journal of Fruit Science;2001-04
9 Yi Ganjun,Yu Xiaoying, Huo Heqiang, Tan Weiping, Chen Dacheng, Huang Ziran, and Huang Bingzhi(Guangdong Fruit Research Institute, Guangdong Academy of Agricultural Sciences ,Guangzhou 510640 China; Hunan Agricultural University ,Changsha4l0128 Chin;Identification and Classification of Banana (Musa AAA Group Cavendish) Varieties by AFLP[J];Journal of Fruit Science;2002-04
10 Yi Ganjun; Huo Heqiang; Cai Changhe; Qiu Yanping; Huang Ziran; Chen Dacheng(1 Pomology Research Institute, Guangdong Academy of Agric. Sci., Guangzhou, 510640;2 Dept. of Sericulture, South China Agric. Univ.;3 Dept. of Horticulture, South China Agric. Uni;DNA Isolation Procedure for AFLP Amplification Reaction of Litchi[J];JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY;1999-03
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