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《Northern Horticulture》 2010-13
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The Transformation System of Oil Sunflower(Helianthus annuus L.)

HUANG Jun-xuan,LI Jian-ke,LI Shuang-yue,LIU Yan-jun,YANG Jing-hui (Horticultural Department of Tianjin Agricultural College,Tianjin 300384)  
In order to establish the system of genetic transformation on oil soybean by Agrobacterium medium transformation of Agrobacterium tumefaciens strains C58C1 with pCB302 vector,the concentration of herbicide,the selection of explants and pre-culture were researched.The results showed that more suitable concentration of herbicide was 3 mg/L,the more desirable explants were stems,the more desirable time of pre-culture was 7 days with highest transformation ratio.
【Fund】: 天津市科委科技支撑资助项目(08ZCKFNC01200) (07ZCKFNC01100);; 教育部留学回国人员科研启动基金资助项目
【CateGory Index】: S565.5
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