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《Northern Horticulture》 2011-01
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Determined and Analyzed of Enzymatic Activity and Protein Electrophoretogram in Hybrid Orchids and Their Hybrid Generation

ZHU Qing-song1,ZHANG Jing-hua2,WANG Guang-dong3,ZHAO Hai-ying4(1.Xinyang Agricultural College,Xinyang,Henan 464000,2.The Bureau of Agriculture of Queshan County,Zhumadian,Henan 463200;3.College of Horticulture;Nanjing Agricultural University;Nanjing,Jiangsu 210095 4.Xinyang Agricultural Science Research Institute;Xinyang,Henan 464000)  
The enzymatic activity of POD,SOD and CAT in hybrid orchids and their hybrid F1 generation were determined,the electrophoretogram of EST and protein were analyzed.The results showed that the POD、SOD and CAT enzymatic activity of the female parents was lower than that of their male parents among parents and their hybrid F1 generation.Among F1 generation,there was significant difference in POD and CAT,while there was no significant difference in SOD.From the electrophoretogram of EST,it could been seen that female parents had three obvious enzymatic bands and male parents had two.At the same time,abundant variations had been seen in hybrid F1 generation.The hybrid F1,which had their parents' features of the enzymatic bands,account for 33.3%.Protein SDS-PAGE' electrophoresis of parents and their hybrid F1 showed that male parents had six more weak bands than female ones.15.8% hybrid F1 generation had two more weak bands than female ones,and the rest of them had the equal number to their female parents.
【CateGory Index】: S682.31
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