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《Northern Horticulture》 2011-06
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Study of Defense Enzymes Activities on Cultivation Chrysanthemum Infected by Chrysanthemum White Rust

ZHU Peng-fang1,2,ZHAO Ni-la1,DUAN Yu-xi2(1.College of Forestry,Shenyang Agricultural University,Shenyang,Liaoning 110866;2.Plant Protection Center for Post-doctoral Studies,Shenyang Agricultural University,Shenyang,Liaoning 110866)  
We identified symptoms by eyes and under microscope,detected four defense enzymes activity through UV-spectrophotometry termly after inoculating the pathogen to different resistant cut-flowers(C039,C002),potted chrysanthemum(B86,B76) varieties.We focused on activity changes of phenylalanine ammonia lyase(PAL),polyphenol oxidase(PPO),and peroxidase(POD) and after inoculation in order to find relationships of different varieties to resistance.The results showed that we had not found any symptoms on disease-resistant cultivarin 15 days but obvious telia on C002 10 days after inoculation,obvious telia on B76 11 days after inoculation.With infection of the pathogen,activity of PAL,PPO and POD increase compared with their contrasts respectively,the amplitude of improvement and variation on activity differ from one type of enzyme to another,for each enzyme,the acitivity of resistant varieties can be increased to a higher extent than susceptible varieties Activities changes of the three defense enzymes were closely associated with resistance to P.horiana in the whole interaction between chrysanthemum and the pathogen.the length of latent period of chrysanthemum white rust varies in varieties,thus,the height and time of defense enzymes' peak can be regarded as an important physiological indicator when the chrysanthemum is infected with chrysanthemum white rust in the early time.
【Fund】: “十一五”国家科技支撑计划资助项目(2006BAD01A18);; 沈阳农业大学博士后研究基金资助项目
【CateGory Index】: S436.8
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