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《Northern Horticulture》 2011-09
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Extraction of Lowmethoxyl Pectin from Grapefuit Peel by Activation Pectin Esterase

GAO Xue,CAI Wei-yu,WU Li-wen,HUANG Wei-huan,CHEN Shao-shuang,ZHANG Xue-ping(Department of Biology,Hanshan Normal University,Chaozhou,Guangdong 521041)  
Lowmethoxyl pectin(LMP)was obtained form fresh grapefruit peel by using Na2CO3 as activator to active pectin esterase in fresh peel.Pectin was de-esterified,then extracted,precipitatied and purified.The results showed that de-esterified temperature,pH level,time,and the percent of Na2CO3 to peel and water were 45℃,pH 7.5,50 min and 0.15%,respectively,and the acid-extraction temperature,pH level and time were 85℃,pH 3.5 and 60 min,respectively.Under this condition,the output rate of pectin was 3.0% with the content of methoxyl below 7%,which was in accordance with the national standard for LMP.
【Fund】: 国家星火计划资助项目(2008GA780034);; 韩山师范学院团队科研资助项目(LT200802) 韩山师范学院“大学生创新性实验计划”资助项目
【CateGory Index】: TQ432.71
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