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Study on the Cultivating Flammulina velutipes (Fr.)Sing·Using Spent Grains of Chinese Spirit

ZHANG Kai-zheng1,2,ZHANG Ze-yan1,SI Xue-qiang1,WANG Lan1,XU De-fu3,ZHANG Wen-xue2(1.School of Biotechnology Engineering,Sichuan University of Science and Engineering,Zigong,Sichuan 643000;2.College of Light Industry,Textile and Food Engineering,Sichuan University,Chengdu,Sichuan 610065;3.Luzhou Laojiao Stock Corporation,Luzhou,Sichuan 646000)  
By the method of the L16(45)orthogonal test of cultivating Flammulina velutipes(Fr.)Sing.using spent grains of chinese spirit,the optimum cultivating conditions of Flammulina velutipes(Fr.)Sing.were studied.The results showed that the pretreatment mode of spent grains was the main factor which affected the cultivation,the additive amount of sawdusts and brans was the secondly factor,then was the sterilization condition and pH of culture medium successively on the cultivation of Flammulina velutipes(Fr.)Sing..The optimum cultivating conditions were A3B4C1D2,which was the washed spent grains were pressed to the water content of 45%,pH of culture medium was 7,the additive amount of sawdusts and brans was 30% and the sterilization condition was 10 h on the ordinary pressure.The results showed that the material percent conversion was 74.9%,it took 30 days that polypropylene bags were fulled with hypha,and the contamination rate was 16.67%.
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