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《Northern Horticulture》 2012-02
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Cloning of the Squalene Synthase Gene 2 cDNA from Eleutherococcus senticosus and Analysis of Its Putative Protein Structure

LONG Yue-hong,XING Zhao-bin,LIANG Neng-song,HE Shan,LI Bao-cai,ZHU Jin-li(College of Life Science,Hebei United University,Tangshan,Hebei 063000)  
Full-length of E.senticosus squalene synthase gene 2 cDNA(GenBank number: JN714465) was cloned by RT-PCR and RACE methods.Sequence analysis revealed a 1 463 bp cNDA containing 10 bp 5′UTR,208 bp 3′UTR and 1 245 bp ORF encoding 414 amino acids.The protein had one Trans_IPPS_HH conserved domain,two specificity target region of phytoene synthase and two thransmembrane helixs.Amino acids comparison showed that the consistence of the protain and SS in other Araliaceae plants was more than 90%.The consistence wrth E.senticosus SS1 was 91.79%.
【Fund】: 国家自然科学基金资助项目(30701086);; 河北省自然科学基金资助项目(C2009001252)
【CateGory Index】: S567.19
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