Establishment of Tissue Culture System of Pinellia ternata(Thunb.) Breit.
TAO Mi-lin,LIU Qing-bo,HUANG Hong-mei(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha,Hunan 410128)
Taking terminal bud and tuber as explants,the optimal conditions for inducing callus from terminal bud,differentiating of adventitious buds from callus,differentiating of adventitious buds from tuber and rooting of test-tube seedlings of Pinellia ternata(Thunb.) Breit.were studied.The results showed that the best medium to induce callus was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L,MS+6-BA 1.0 mg/L+NAA 0.2 mg/L was the best differentiation adventitious bud medium from terminal bud callus or tuber,the optimal culture medium was MS+2,4-D 2.0 mg/L+KT 1.0 mg/L for rooting.