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《Northern Horticulture》 2014-10
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Study on Pathogen Identification and Biological Characterstics of the Leaf Spot of Calotropis gigantean

XIE;Mei-hua;LI Lin;LI Xue-ling;YANG Hai-yan;CHEN Hua-hong;WANG Zhen-ji;Department of Chemistry and Life Science,Chuxiong Normal University;Yunnan Province Applied Biology Key Laboratory of University;Information Center,Chuxiong Pharmaceutical College;  
One fungal strains was separated from the leaf spot of Calotropis gigantean,noted for CX008.It was identified through the combination of morphological observation and modern molecular biological technique,the culture traits of it were studied based on the morphological characteristics.The results showed that:the pathogen is Alternaria tenuissima(Nees ex Fr.).The fungi was able to use 7kinds of carbon and nitrogen sources.The best carbon and nitrogen source for its growth were glucose and beef extract respectively.The mycelial could grow at 15~40℃,the approprate temperature for its growth was 25~30℃,mycelial stopped growing at 5℃,10℃and 45℃.Its spore was able to germinate at 15~ 40℃,the approprate temperature was 28℃,it could not germinate when temperature exceed 40℃.12hlight and dark alternative was helpful to mycelial growth,photoperriod had little influence on spore germination.In laboratory test,there were inhibitory action for 5kinds broad-spectrum fungicide on mycelial growth.Iprodione was the best getrmicides on the tested,then Metalaxyl mancozeb,Chlorothalonil and Mancozeb as follow,the worst was Fenaminosulf.
【Fund】: 国家自然科学基金资助项目(31300370);; 云南省应用基础计划资助项目(2011FZ186);云南省应用基础计划项目青年资助项目(2012FD049);; 云南省重点建设学科楚雄师范学院重点建设学科基金资助项目(05YJJSXK03);; 云南省高校科技创新团队支持计划资助项目(IRTSTYN);; 楚雄师范学院院级资助项目(05-YJRC04);; 楚雄师范学院大学生创新创业训练计划资助项目(2013cxcy04)
【CateGory Index】: S432.42
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