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Effect of Alternating Temperature on the Spore Sprouting and the Mycelium Growing of the Military Standard Molds

HU Shang-qin(College of Life Science,Chongqing Normal University,Chongqing 400047,China)  
In order to clarify the effect of alternating temperature on the spore sprouting and the mycelium growing of the military standard molds,five kinds of the molds were separately vaccinated in the PDA solid and liquid media by the slide and liquid shake raise methods.The sprouted spore and the grown mycelium were sampled,observed and measured once every 30 min at the alternating temperatures of(30±1)℃ and(25±1)℃,the humidity of(95±5)%,and compared with those sprouted and grown at constant temperature.Under the condition of the alternating temperature,the experimental results show that the mold vitality is improved,the spore dormant state is broken to the advantage of the spore sprouting and the mycelium growing;the spore sprouting speed is quicker than that at constant temperature,the required time is shorter by 40~50 min;the mycelium average length is 1.06~1.13 times as much as that at constant temperature;the mycelium biomass average is 1.08~1.17 times as much as that at constant temperature.The experiment provides a reliable theory basis for adopting the alternating temperature condition in the military standard molds.
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