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《Chinese Journal of Zoology》 2002-02
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Progresses on the Methods for Evaluating the Mammalian Sperm Quality

HUO Li-Jun YANG Zeng-Ming(College of Life Science, Northeast Agricultural University Harbin 150030,China)  
All aspects of functions in spermatozoon reflect its fertility. The fluorescent probes for assessing the integrity of sperm plasma membrane include SYBR-14, SYTO-17, carboxyfluorescein diacetate (CFDA), carboxy dimethyl fluorescein diacetate (CDMFDA), calcein acetyl methyl ester (CAM), propidium iodide (PI), Hoechst 33258, and Hoechst 33342, of which SYBR-14/PI is the best method. The fluorescent probes for determining sperm mitochondria activity include JC-1, Mitotracker green FM(MITO), and rhodamine123 (Rh123), of which JC-1 is better than MITO and Rh123. Acrosome integrity of spermatozoa can be examined with PNA-FITC, PSA-FITC, LYSO-G and chlortetracycline (CTC). CTC is also used to identify whether sperm is capacitated. Moreover, sperm quality can also be predicted through binding oocyte, penetrating the zona-free oocyte, and fertilizing oocytes.
【Fund】: 国家杰出青年科学基金资助项目 (No.3982 5 12 0 )
【CateGory Index】: S814
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