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《Chinese Journal of Zoology》 2003-03
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Effect of Cu~(2+) on the Culture of Haemocytes from Macrobrachium nipponense

WANG Jun-Xia① WANG Wei-Na ①② WANG An-Li② WANG Ya-Bin③(①College of Life Science,Hebei University, Baoding 071002; ②College of Life Science, South China Normal University, Guangzhou 510631; ③Environment Monitor Bureau in Baoding, Baoding 071001, China)  
Haemocytes of Macrobrachium nipponense cultured in vitro were studied. It was found that satisfactory results could be obtained using medium 199 supplemented with 15% fetal bovine serum (FBS), 100?IU/ml Penicillin, 100?μg/ml streptomycin osmolarity adjusted to 472?mmol/L. The effect of Cu 2 on subcultured haemocytes was studied. Basal medium supplemented with Cu 2 2?μg/L enhanced the growth of haemocytes and improved their total phosphate and AKP activity.
【Fund】: 河北省重大科技攻关计划项目 (No.85- 93 -2 9)
【CateGory Index】: S966.12
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