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《Chinese Journal of Zoology》 2003-03
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Long PCR Technique and its Applications in Zoological Study

YE Wei-Ping HUANG Yuan (College of Life Science, Shaanxi Normal University, Xi′an 710062, China)  
The advent of long PCR has proven to be a major advance in PCR technology. This article reviews the origins, special requirements with respect to templates, primers, DNA polymerases, and reaction conditions, of long PCR. Recent developments in long PCR, including long range-inverse PCR (LR-IPCR), endonuclease-mediated long PCR, long RT-PCR and LDD-PCR, are introduced. Finally, applications of long PCR in zoological study are summarized with respect to genome sequencing and mitochondrial genome research, pathogenetic diagnosis, differential gene expression, pathogenetic virus research and analysis of genetic polymorphism.
【Fund】: 国家自然科学基金项目 (No .NSFC30 0 70 114);; 陕西师范大学2 0 0 2年研究生培养创新基金资助
【CateGory Index】: Q95-3
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