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《Chinese Journal of Zoology》 2005-01
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Isozyme Changes in Different Stage Embryos and Aborted Embryos in Eriocheir sinensis

JIA Shou-Ju YING Xue-Ping CHEN Yan-Le YING Jian-Ping(School of Biological and Environmental Science, Wenzhou Normal College, Wenzhou 325027, China)  
Six isozymes, LDH, ADH, MDH, EST, AMY, and POD, in different stage embryos and aborted embryos in Eriocheir sinensis were investigated with polyacrylamide gel electrophoresis. The results showed that the activities of LDH, ADH, MDH, EST and AMY were different in embryos at different developmental stages. LDH isozyme could not be detected in fertilized eggs, while four isozyme bands emerged in cleavage and blastulation stage embryos and two isozyme bands in nauplius and zoaea stages; ADH isozyme was expressed in all stages of embryonic development, but there were differences in enzyme bands and enzyme activities. No MDH enzyme bands were observed in fertilized eggs and at zoaea stages, while most bands and the strongest activities emerged at cleavage stages. With the development, the MDH enzyme bands and activities became weakened. The changes in EST isozyme were more complicated, with sudden disappearance at blastulation stage. The band number of AMY, both (α-AMY and R-AMY) decreased from fertilized eggs to larver stage.The POD activity could not be detected in all developmental stages. The enzyme activities and bands of the second spawning embryos were different from those of the first cleavage stageembryos. The ADH, LDH, and AMY isozyme bands of aborted embryos had slight differences compared to the first spawning embryos at different developmental stages, while MDH, EST isozyme bands decreased dramatically in aborted embryos.
【Fund】: 温州市科技局基金资助项目 (No.N2 0 0 0A2 6 ) ;; 乐清市科技局基金资助项目
【CateGory Index】: Q593
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【Citations】
Chinese Journal Full-text Database 10 Hits
1 CHENG Yong Xu (Department of Oceanography, Xiamen University,Xiamen\ 361005,China) DU Nan Shan\ LAI Wei (Department of Biology, East China Normal University, Shanghai\ 200062,China);ON THE ULTRASTRUCTURE OF YOLK LIPID DISTRIBUTION AND ITS CHANGES DURING THE CHINESE CRAB, ERIOCHEIR SINENSIS OVARIAN MATURATION AND EMBRYONIC DEVELOPMENT[J];CHINESE JOURNAL OF ZOOLOGY;1999-01
2 Cheng Huizhen Yao Bo Lin Huixian Ren Shaofen Cai Shaowei (Dept of Biology, Guangzhou Teachers College);A Comparative Study on Esterase Isozyme of Some Tissues and Cells from Macrobrachium Rosenbergii Before and After Oviposition[J];JOURNAL OF GUANGZHOU NORMAL UNIVERSITY;1997-01
3 TIAN Hua\|Mei\ ZHAO Yun\|Long\ LI Jing\|Jing\ CUI Bao\|Jun (School of Life Sciences, East China Normal University\ Shanghai\ 200062, China);Biochemical Changes during Embryonic Development in the Crab Eriocheir sinensis[J];Chinese Journal of Zoology;2002-05
4 Zhao Yunlong Du Nanshan Lai Wei (Department of biology, East China Normal University, Shanghai 200062);EFFECTS OF DIFFERENT GRADIENT TEMPERATURE ON EMBRYONIC DEVELOPMENT OF THE CHINESE MITTEN-HANDED CRAB, Eriocheir sinensis(CRUSTACEA, DECAPOD)[J];Zoological Research;1993-01
5 LU Jian\|ping, JIANG Nai\|cheng (College of Life Science, Zhejiang University,Hangzhou 310012, China);The isozyme patterns in embryo development of the freshwater prawn Macrobrachium rosenbergh[J];DONGHAI MARINE SCIENCE;2000-03
6 WangDanYu Weijun Research Institute for Freshwater Fisheries of Liaoning Province;A Comparative Study on the IsozymesinEriocheir Sinensis From Chang Jiang and From Liao He Rivers[J];JOURNAL OF LIAONING UNIVERSITY((NATURAL SCIENCE EDITION));1995-04
7 Wu Lizhao and Wang Zuxiong (Institute of Hydrobiology,The Chinese Academy of Sciences,Wuhan,430072);STUDIES ON THE EXPRESSIONS AND REGULATIONS OF ISOZYMIC GENES IN SILVER CARP (HYPOPHTHALMICHTHYS MOLITRIX)DURING ONTOGENESIS[J];ACTA HYDROBIOLOGICA SINICA;1997-01
8 YING Xue-Ping1,LUO Yuan-Yuan2 and ZHANG Yong-Pu1 (1. Department of Biological and Environmental Science.Wenzhou Normal College,Wenzhou 325027; 2.College of Life Science,China Institute of Metrology,Hangzhou 310012);A COMPARATIVE STUDY ON THE COMPOSITION OF AMINO ACID AND FATTY ACIDIN FERTILIZED EGGS AND LOST EGGS OF ERIOCHEIR SINENSIS[J];Acta Hydrobiologica Sinica;2004-04
9 LI Guang-li and ZHU Chun-hua (Fisheries College of Zhanjiang Ocean University, Zhanjiang, 524025);STUDIES ON ISOZYMES DURING EARLY ONTOGENY OF MACROBRACHIUM ROSENBERGII[J];Acta Hydrobiologica Sinica;2001-04
10 Li Chunhou; Zhong Zhenru and Chen Min(South China Sea Fisheries Institute, CAFS, Guangzhou 510300);ISOZYME CHANGES DURING EARLY ONTOGENY OF PENAEUS MONODON[J];JOURNAL OF FISHERIES OF CHINA;1994-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Xu Ping (Dept. of Biology , Chongqing Teachers College , Chongqing , 630047) Yu Chunfang (College of Animal Sciences and Veterinary Medicne , Southwest Agicultural University , Chongqing , 630716);Electrophoresis Analysis of LDH Isoenzymes and Proteins in the Serum of Rabbits[J];JOURNAL OF CHONGQING TEACHERS COLLEGE(NATURAL SCIENCE EDITION);1997-02
2 Lu Wenhuang and Liao Guohua(Sanming Reanrch Institute of Forestry Science,Sanming 365000);Research on the two kinds of bamboo shoots[J];FUJIAN JOURNAL OF AGRICULTURAL SCIENCES;1996-02
3 Fan Yirong;MaoYingchun;Li Lanying;Dai Jianliang;Liu Shifang(Zhejiang Forestry College,Lin'an 311300, P. R. China);Study on the Genetic Variation of Enzyme Genes in Pinus Taiwanensis Population[J];JOURNAL OF NORTHEAST FORESTRY UNIVERSITY;1999-03
4 LIU Cun-qi,WANG Wei-wei,ZHANG Ya-juan(Department of Life Science,Hebei University,Baoding 071002,China);Advance on Superoxide Dismutase in Aquatic Organisms[J];Fisheries Science;2005-11
5 Li Jun Zhong Zhangcheng (Dept.of Life Science,Shouthwest Normal University,Chongqing,400715);An Analysis of POD Isoenzyme of Leaves of Diffenent Environmental Plants of Orychophragmus Violaceus[J];JOURNAL OF CHONGQING TEACHERS COLLEGE(NATURAL SCIENCE EDITION);1998-03
6 OU Bing Qing REN Ji Jun HE Li Lan(Foshan University, Nanhai,528231);Application of Peroxidease in the classification of Pumpkin[J];Journal of Foshan University(Natural Science Edition);2002-03
7 HUANG Jian-buo 1,OU Bing-qing 2,HE Li-lian 1(1.Agronomy Department,Foshan University,Nanhai 528231,China; 2.Chemistry and Chemical Engineering Department,Foshan University,Foshan 528000,China);Research of peroxidase in banana tissue seedling[J];Journal of Foshan University(Natural Science Edition);2002-04
8 XUE Jun Zeng (Department of Biology,Hangzhou Normal College,Hangzhou 310036,China) DU Nan Shan LAI Wei (Department of Biology,East China Normal University,Shanghai 200062,China)$$$$;Studies on the Histology of Early Embryonic Development of Portunus trituberculatus[J];Zoological Research;2001-01
9 Chen Zhenggu;Cheng Beijiu;Sun Wenhu;Liu Xiaogang Laboratory of Laser & Breeding, Anhui Agricultural College, Hefei;BIOLOGICAL EFFECTS OF WATER IRRADIATED WITH CO_2 LASER IN COTTON[J];Journal of Anhui Agricultural University;1991-03
10 XUE Jun Zeng (Department of Biology,Hangzhou Normal College,Hangzhou 310036,China) DU Nan Shan LAI Wei (Department of Biology,East China Normal University,Shanghai 200062,China);Genesis and Development of the Digestive System of the Swimming Crab,Portunus trituberculatus,in the Embryo[J];Zoological Research;2001-05
China Proceedings of conference Full-text Database 9 Hits
1 ;Electrophoretic analysis of the EST isozymes and soluble protein of several species in Fusarium[A];[C];2006
2 Chen Hui,Chen Xiaoling, Chen Longqing, Lu Xinxiong Key Laboratory for Crop Germplasm and Biotechnology of Agricultural Ministry/The National Key Facility for Crop Gene Resources and Genetic Improvement,NFCRL Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; College of Horticultural and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China;Cryopreservation of shoot tips from in vitro plants of cut flower of lily (Lilium L.) by vitrification method[A];[C];2006
3 Yang Qingzhen Sun Haiyan Ji Lan (College of Horticulture,Shanxi Agricultural University,Taigu 030801);Preliminary Study of Isoperoxidase in Hazelnut[A];[C];2003
4 WANG Xiao-Mei, WANG Qian, DAI Wei, LI Xiao-Dong, CHEN Shuai-Jun, MA Cheng-Xue Key laboratory of Aquatic Ecology & Aquaculture of Tianjin, Department of Fisheries Science, Tianjin Agricultural College, Tianjin 300384, China Panjin Guanghe Fisheries Co. Ltd., Panjin 124200, Liaoning Province, China Dalian Fisheries University, Dalian 116023, Liaoning Province, China;RFLP analysis of mitochondrial 12S - 16S rDNA on mitten crabs[A];[C];2005
5 Zhang Jie Chen Liqiao Zhou Zhongliang Tang Sixian;Study on the Developmental Genetics of Isozymes in Chinese Mitten-handed Crab I.Discussion on Isozymes in Various Tissues of Chinese Mitten-handed Crab from Changjiang River[A];[C];1996
6 ZHAO Yan-min,YAO Jun-jie,ZHAO Yun-long,AN Chuan-guang,DUAN Xiao-wei School of Life Science,East China Normal University,Shanghai,200062,China;The changes of protein and amino acid composition during embryonic development in Macrobrachium nipponense[A];[C];2005
7 WANG Gui-ling, XU Hang, CHEN Zhi-guo, SHEN Wei-rong College of Aqua - life Science and Technology, Shanhai Fisheries University, Shanghai, ,200090 School of life science, Ningxia University, Yinchuan 750021;Studies on four isozyme systems in sex reversal process of Monopterus albus[A];[C];2005
8 YANG Xiao-zhen~1 WU Xu-gan~1 YAO Gui-gui~1 CHENG Yong-xu~1 WANG Chun-lin~2 (1.Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Fisheries University,Shanghai 200090,China; 2.College of Life Science and Biotechnology,Ningbo University,Ningbo 315211,China);Ultrastructure of Oocytes and Follicular Cells of Portunus trituberculatus during the First Reproductive Cycle[A];[C];2008
9 MENG Fan-Li CHEN Li-Qiao ZHAO Yun-Long(East China Normal University, Shanghai 200062, China);THE METHODS OF THE STUDY ON THE EMBRYONIC DEVELOPMENT OF CRUSTACEA[A];[C];1999
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Du NANSHAN LAI WEI XUE LUZHEN(Department of Biology, East Chita Normal University, Shanghai);ACROSOME REACTION OF THE SPERM IN THE CHINESE MITTEN-HANDED CRAB, ERIOCHEIR SINENSIS (CRUSTACEA, DECAPODA)[J];Acta Zoologica Sinica;1987-01
2 Zhao Yunlong Du Nanshan Lai Wei (Department of biology, East China Normal University, Shanghai 200062);EFFECTS OF DIFFERENT GRADIENT TEMPERATURE ON EMBRYONIC DEVELOPMENT OF THE CHINESE MITTEN-HANDED CRAB, Eriocheir sinensis(CRUSTACEA, DECAPOD)[J];Zoological Research;1993-01
3 Du Nanshan Lai Wei (Department of Biology, East China Normal University, Shanghai) Xue Luzhen (Shanghai Institute of Entomology, Academia Sinica, Shanghai);THE CHROMOSOMES OF THE CHINESE MITTEN-HANDED CRAB, ERIOCHEIR SIN EN SIS (CRUSTACEA, DECAPODA)[J];Zoological Research;1986-03
4 Wang Shuying (Wuxi University of Light Industry,Wuxi 214036) Chen Hui (Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences,Wuxi 214081);Determination of Amino Acids in Different Part of Erocheir sinensis[J];AMINO ACIDS & BIOTIC RESOURCES;1999-02
5 ZHAO Yun Long\ WANG Qun\ DU Nan Shan\ LAI Wei (East China Normal University,Shanghai\ 200062,China);EMBRYONIC DEVELOPMENT OF THE GIANT FRESHWATER PRAWN, MACROBRACHIUM ROSENBERGII (CRUSTACEA:DECAPODA): Ⅰ.MORPHOGENESIS OF EXTERNAL STRUCTURES OF EMBRYO[J];ACTA ZOOLOGICA SINICA;1998-03
6 DU NANSHAN XUE LUZHENG LAI WEI (Department of Biology, East China Normal University, Shanghai);HISTOLOGY OF THE MALE REPRODUCTIVE SYSTEM IN ERIOCHEIR SINENSIS (DECAPODA, CRUSTACEA)[J];Acta Zoologica Sinica;1988-04
7 PENG Yu ① HU Cui ② ZHAO Jing Zhao ① CHEN Jian ① (① School of Life Science, Hubei University, Wuhan?430062, China) (② Department of Plant Protection, Zhejiang University, Hangzhou?310029, China);CHANGES OF THE MORPHOLOGICAL AND CHEMICAL SUBSTANCES OF THE EGG OF PIRATA PIRATICUS DURING EMBRYONIC DEVELOPMENT[J];Acta Zoologica Sinica;2001-02
8 CHENG Yong Xu (Department of Oceanography,Xiamen University ,Xiamen 361005,China) DU Nan Shan \ LAI Wei (Department of Biology,East China Normal University,Shanghai\ 200062,China);LIPID COMPOSITION IN HEPATOPANCREAS OF CHINESE MITTEN CRAB ERIOCHEIR SINENSIS AT DIFFERENT STAGES[J];ACTA ZOOLOGICA SINICA;1998-04
9 YANG Wan\|xi, ZHOU Hong (College of Life Science, Zhejiang University, Hangzhou, 310012, China);Primary study on the histology of exoskeleton of female pleopod in Eriocheir sinensis[J];DONGHAI MARINE SCIENCE;2000-03
10 Du Nanshan, Xue Luzheng and Lai Wei(East China Normal University, Shanghai);STUDIES ON THE SPERM OF CHINESE MITTEN-HANDED CRAB, ERIOCHEIR SINENSIS (CRUSTACEA,DECAPODA) II. SPERMATOGENESIS[J];Oceanologia Et Limnologia Sinica;1988-01
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