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《Chinese Journal of Zoology》 2006-06
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A Modified Method for Evaluating Phagocytosis and Encapsulation in Haemocytes of Chlamys farreri

HAN Qiang SUN Hu-Shan WANG Yi-Yan RAN Zhen HUANG Qing-Rong(College of Life Sciences,Ludong University,Yantai 264025,China)  
The aim of this study is to establish a kind of fast and accurate method for evaluating phagocytosis and encapsulation of haemocytes in vitro.The haemolymph of scallop Chlamys farreri was aspirated from adductor muscle with a syringe.Haemocytes were incubated with bacilli or camellia farinas for 30 min at room temperature,then dyed with Acridine Orange(AO).The phagocytosis and encapsulation of haemocytes were observed with a fluorescent microscope.The results showed that haemocytes were green and the bacilli or camellia farinas were red under the fluorescent microscope,and that the color contrast between haemocytes and baciluses or camellia farinas was great,which makes the evaluation of phagocytosis or encapsulation easier and more accurate.
【Fund】: 国家“九七三“计划项目(No.G1999012005);; 鲁东大学大学生科技创新基金项目
【CateGory Index】: Q46
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