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《Chinese Journal of Zoology》 2008-06
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Genetic Diversity of Sus scrofa in Southern Area of Yunnan Province Based on Microsatellite Markers

HUO Jin-Long~ ①② MIAO Yong-Wang~ ②③ HUO Hai-Long~② LI Da-Lin~④LIU Li-Xian~⑤ LIU Jian-Ping~⑥ LI Yun-Hui~⑦(① Key Laboratory of Banna Mini-pig Inbred Line of Yunnan Province,Yunnan Agricultural University,Kunming 650201; ② Faculty of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201; ③ Laboratory for Conservation and Utilization of Bio-resources,Yunnan University,Kunming 650091; ④ Domestic Animal Breeding and Crossbreed-improvement Station of Yunnan Province,Kunming 650021; ⑤ Department of Husbandry and Veterinary Medicine,Yunnan Agricultural Vocational Technical College,Kunming 650212; ⑥ Xishuangbanna Animal Husbandry and Veterinary Station of Yunnan Province,Jinghong 666100; ⑦ Mengla County Agricultural Bureau of Yunnan Province,Mengla 666300,China)  
Wild Boar (Sus scrofa) is not only the ancestor of domestic pigs,but also important genetic resources and the constituent of biological diversity.In order to further get insight into the genetic variability in its population and provide the genetic background and guidance for its conservation and utilization,the microsatellite DNA polymorphisms for 65 individuals sampled from the southern area of Yunnan province were assayed by using PCR and polyacrylamide gel electrophoresis.Of 76 microsatellite loci tested which are located on swine’s 19 chromosomes,a total of 349 alleles were detected,and the number of alleles varied from 3 to 9 and the number of effective alleles from 1.683 5 to 8.066 7, with the mean value of 4.592 1±1.145 2 alleles and 3.709 9±0.990 4 effective alleles per locus.The observed heterozygosity,expected heterozygosity and polymorphism information content were estimated to be 0.949 2±0.113 5,0.711 6±0.078 7,and 0.663 4±0.092 1,respectively.The results show that the genetic diversity in the population of the Wild Boar in Yunnan Province is high.
【Fund】: 云南省教育厅科学研究基金项目(No.07Y40124);; 国家自然科学基金项目(No.30660024);; 云南省应用基础研究重点项目(No.2007C0003Z);; 云南省应用基础研究计划面上项目(No.2006C0034M)
【CateGory Index】: Q953
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1 HUO Jin-long1, HUO Hai-long1, MIAO Yong-wang1,2,*, LI Fu-quan3, LIU Li-xian4; WU Ge-min1, OUYANG Yi-na1, QIAN Kun5 (1. Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China; 2. Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091, China; 3. Department of Biotechnology, Neijiang Vocational & Technical College, Neijiang 641100, China; 4. Department of Husbandry and Veterinary, Yunnan Agricultural Vocational Technical College, Kunming 650212, China; 5. Huzhou Normal College,Huzhou 313000, China);Genetic Diversity of 76 STR Loci in the Dahe Pig[J];Zoological Research;2009-01
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1 SUN Xue-lin~ 1 , YAO Shao-kuan~ 2 , ZHANG Qin~ 3 , LIU Pei-qiong~ 2 (1.Congjiang County Farming Bureau, Guizhou 557400; 2.College of Animal, Guizhou University, Guiyang 550025; 3.College of Animal, China Agricultural University, Haidian, Beijing 100094, China);Analysis on Genetic Variability of Xiang-pig by Using Microsattelite Markers[J];Guizhou Agricultural Sciences;2005-06
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1 JIANG Mei1,YANG Yong2,YANG Si-biao3,LI Fu-quan4,ZHAO Jun5,WAN Jin-quan1(1.Dali University,Dali,Yunnan,671003,China;2.Yuecheng Animal Husbandry and Veterinary Station,Ziyang,Anyue,Sichuan,642350,China;3.Institute of Yunnan Animal Husbandry and Veterinary,Kunming,Yunnan,650224,China;4.Neijiang Vocational and Technical College,Neijiang,Sichuan,641100,China;5.Mufeng Animal Health Care Kunming Branch,Kunming,Yunnan,650000,China);PCR Amplification and Polymorphism Analysis of Porcine Phosphoipid Transfer Protein(PLTP) Gene[J];Progress in Veterinary Medicine;2009-06
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