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《Chinese Journal of Zoology》 2011-03
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Comparative Analysis of mtDNA Gene between Two Rare Schizothoracine Fishes

YANG Tian-Yan MENG Wei ZHANG Ren-Ming GUO Yan HAI Sa XIE Chun-Gang Xinjiang Fisheries Research Institute,Urumqi 830000,China  
The mtDNA COⅠ(624 bp),Cyt b(712 bp) and D-loop(457 bp) gene fragments of Aspiorhynchus laticeps and Schizothorax biddulphi were sequenced by PCR respectively and sequence comparisons were made to identify differentiation.The percentage of A+T content was higher than that of G+C content in three gene fragments.There was a strong bias against G content,especially in the third codon positions of protein-coding genes(COⅠ and Cyt b).Sequence analysis indicated that the variation of intra-species was low and the nucleotide substitution rate of three gene fragments arranged from high to low was D-loopCyt bCOⅠ.The estimated divergence time between two Schizothoracine species based on Cyt b gene was about 3.57 MaBP in Pliocene,which was earlier than the specialized grade and high specialized grade species.Because the Tarim River lies in the northern edge of the Qinghai-Tibet Plateau and is geographical development as a part of the Tibetan Plateau,the uplift of Qinghai-Tibet Plateau influences the evolution of Schizothoracine species in Xinjiang to some extent.
【Fund】: 国家自然科学基金项目(No.31060356);; 新疆维吾尔自治区自然科学基金项目(No.2009211A30)
【CateGory Index】: S917.4
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