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《Acta Scicentiarum Naturalum Universitis Pekinesis》 1990-06
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Preparation of Monoclonal Antibody Against Human Urokinase by Using Intrasplenic Primary Immunization

LI Erqiu, ZHU Xiaomao, XU Chang fa (Department of Biology)  
Using a novel immune technique-intrasplenic primary immunization,one hybridoma line 3C6 has been established, which secreted monoclonal antibody against urokinase by fusion between SP2/0 myeloma cells and spleen cells from a BALB/c mouse immunized with urokinase. Monoclonal antibody (McAb) 3C6 strongly bound to urokinase and showed no crossreaction with trypsin and plasminogen and some crossreaction with chymotrypsinogen and chymotrypsin. Immunoblotting of urokinase showed that McAb 3C6 recognized either the 54,000 dalton or the 33,000 dalton urokinase chain. Subclasses of McAb 3C6 is IgM.
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【References】
Chinese Journal Full-text Database 1 Hits
1 Ren Yuhong(Department of Biochemistry, Yantai University, Yantai 264005) Zhao Chunmei Li Erqiu Xu Changfa(Departmnet of Biochemistry, Molecular Biology, Peking University, Beijing 100871);Preparation of Monoclonal Antibodies Against Human Urokinase[J];JOURNAL OF YANTAI UNIVERSITY;1997-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Cao Chunzhang 1,Bu Lisha 1,Zhao Hong 1,Wang Weizhong 1,Mao Huawei 2 (1\ Central Laboratory,The Third Clinical College,NBUMS;2\ The Hospital of Changchun Taxation College);Preparation of monoclonal antibodies against urokinase-type plasminogen activator (uPA) and clinical application in the diagnosis of cancer[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1999-06
2 ZHENG Ping 1, ZOU Rou-jin 1, LIU Yi-xun 2 (1.Kunming Institute of Zoology,the Chinese Academy of Sciences,Kunming,Yunnan 650223,China;2.State Key Laboratory of Reproductive Biology,Institute of Zoology,the Chinese Academy of Sciences,Beijing 100080;Possible Involvement of Plasminogen Activator in the Acquirement of Sperm Progressive Motility and the Regulation of PA and PAI-1 mRNA Expressions in Epididymis,Prostate and Seminal Vesical in Rhesus Monkey[J];Zoological Research;2002-01
3 CHEN De-yu,Liu Sheng-jie,ZHU Mao-ying(Department of Biology,Fuyang Normal College,Fuyang,236041,China);Advances in Male Contraceptive Vaccine[J];Journal of Fuyang Teachers College(Natural Sciences);2006-04
4 DING Xiao-fang, XIONG Cheng-liang Reproductive Medicine Center, Family Planning Research Institute, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, China;Effects of Urokinase-type Plasminogen Activator on Chemotactic Responses of Spermatozoa in vitro[J];Nationlal Journal of Andrology;2005-06
5 WANG Ling,GUAN Huang-tao,TIAN Yong-hong,XIONG Cheng-liangReproductive Medicine Center,Family Planning Research Institute,Tongji Medical College,Huazhong University of Science & Technology,Wuhan,Hubei 430030,China;Urokinase-type Plasminogen Activator and Urokinase-type Plasminogen Activator Receptor in the Seminal Plasma and Sperm of Fertile and Oligoasthenozoospermia Males[J];National Journal of Andrology;2006-09
6 DING Xiao-fang1,2,SHANG Xue-jun3,LI Hong-gang1,GUAN Huang-tao1,XIONG Cheng-liang11.Family Planning Research Institute,Tongji Medical College,2.Center of Reproductive Medicine,Union Hospital,Huazhong University of Science and Technology,Wuhan,Hubei 430030,China;3.PLA Research Institute of Clinical Laboratory Medicine,Nanjing General Hospital of Nanjing Command,Nanjing,Jiangsu 210002,China;Effect of Urokinase-Type Plasminogen Activator on the Mitochondrial Membrane Potential of Mouse Capaciated-Spermatozoa in vitro[J];National Journal of Andrology;2007-05
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10 ZHENG Ping 1, ZOU Ru Jin 1, LIU Yi Xun 2,* (~ 1Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223; ~ 2State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080);Source of plasminogen activator in rhesus monkey semen and its possible role in sperm capacitation[J];Acta Physiological Sinica;2001-01
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