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《Acta Scicentiarum Naturalum Universitis Pekinesis》 1993-04
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Application of Single Sweep Polarography in the Study of Metallothionein

TIE Feng, WANG Wenqing (Department of Technical Physics, Peking University)PAN Aihua, RU Binggen, LI Lingyuan (Department of Biology, Peking University)  
Determination of zinc and cadmium by polarographic adsorptive wave were applied in the study of metallothionein (MT). Zn was released from MT with nitric acid, and then determined in the pH 5 supporting electrolyte containing α, α'-dipyridyl by single sweep polarography. The measurement was used for Zn monitoring during the purification of Zn-MT, a potential medicine, from Zn-induced rabbit liver. In the solution of 0.02 mol/L HC1-0.02 mol/L KI, the Cd in Cd-MT was polarographicly detected, which was used to Cd/hemoglobin saturation for the determination of MT in animal tissues. The assay was capable of detecting 5μg MT/g wet tissue. The recovery is in the range of 90-110%.
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