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《Acta Scicentiarum Naturalum Universitis Pekinesis》 2001-02
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Studies on Amplified Assay of Surface Plasmon Resonance Immunosensing

PEI Renjun WANG Erkang YANG Xiurong (Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry, Chinese Academy of Sciences,Changchun,130022)  
Surface plasmon resonance(SPR)immunosensor is a relatively new immunoassay technique and has been receiving more and more attention in recent years.However,a major disadvantage of SPR for bioanalytical applications is that low concentration or low molecular mass analytes could not be detected directly.Therefore,it is a challenging task to develop strategies for improving the detection limit/sensitivity of SPR.In this paper,authors present a novel strategy for improving the sensitivity of SPR immunosensing using streptavidin biotinylated antibody complex.It is proven that the amplification strategy causes a dramatic improvement of the detection sensitivity.This amplification strategy is based on the construction of a molecular complex between streptavidin and biotin labeled protein.The complex can be formed in a cross linking network of molecules so that the amplification of response signal will be realized due to the big molecular size of complex.
【Fund】: 中国博士后科学基金;; 中国科学院王宽诚博士后工作奖励基金资助项目
【CateGory Index】: O646
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