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《Acta Scicentiarum Naturalum Universitis Pekinesis》 2001-04
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Cre/loxP-mediated Deletion of Transgene in Transgenic Tobacco

ZHEN Wei WANG Yangming\ DING Wei\ CHEN Xi\ Hu Yuanlei\ LIN Zhongping (National Laboratory of Protein Engineering and Plant Genetic Engineering,Peking University,Beijing,100871)  
In the present study,we develop an efficient method to be used to examine recombination efficiency of transgenic plantlet.Two constructs,one containing Cre recombinase under the control of 35 S promoter (p35S/Cre) and the other containing a \%uidA\% gene driven by 35 S promoter flanked by loxP site (loxP/35S/GUS/loxP),were simultaneously introduced into tobacco by cotransformation method.The results show that Cre loxP system can precisely and efficiently direct deletion of transgene based on Gus assay,PCR analysis and sequence of recombination DNA fragment,but also find that incomplete deletion exist in partial plant.
【Fund】: 高等学校博士学科点专项科研基金资助项目
【CateGory Index】: Q784
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【References】
Chinese Journal Full-text Database 2 Hits
1 QIU Shu-ping1,2,CHEN Zai-jie1,WANG Feng1(1.Fujian Key Laboratory of Genetic Engineering for Agriculture,Fujian Academy of Agricul-tural Sciences,Fuzhou,Fujian 350003,China;2.College of Life Sciences,Fujian Normal University,Fuzhou,Fujian 350007,China);Application of Cre/loxp site-specific recombination technology on transgenic plants[J];Fujian Journal of Agricultural Sciences;2008-02
2 PENG Xiang-lei, LIANG Bin, CHEN Ming, HU Yuan-lei and LIN Zhong-ping (National Key Laboratory of Protein Engineering and Plant Genetic Engineering , Peking University , Beijing 100871 , P. R. China);Copper-Controllable, Site-Specific DMA Excision in Transgenic Plants[J];中国农业科学(英文版);2003-06
【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 QIU Shu-ping1,2,CHEN Zai-jie1,WANG Feng1(1.Fujian Key Laboratory of Genetic Engineering for Agriculture,Fujian Academy of Agricul-tural Sciences,Fuzhou,Fujian 350003,China;2.College of Life Sciences,Fujian Normal University,Fuzhou,Fujian 350007,China);Application of Cre/loxp site-specific recombination technology on transgenic plants[J];Fujian Journal of Agricultural Sciences;2008-02
2 WANG Xiu-zhi~1 ZHANG Hong-yu~2 WANG Xu-dong~2 WU Xian-jun~2(1.HLJ August First Land Reclamation University,Daqing,Heilongjiang 163319;2.Rice Research Institute of Sichuan Agricultural University,Wenjiang,Sichuan 611130);A PRELIMINARY STUDY ON Agrobacterium Tumefaciens-MEDIATED CO-TRANSFORMATION OF DOUBLE GENES IN RICE[J];Journal of Nuclear Agricultural Sciences;2009-01
3 WANG Yong 1, LIN Zhong ping 1, WANG Guan lin 2 (1.National Lab of Protein Engineering and Genetic Engineering of Plant, Peking University, Beijing 100871,China; 2.College of Life Sciences, Liaoning Normal University, Dalian 116029,China);Transgenic Approach to Male Sterility of Plants[J];Journal of Liaoning Normal University(Natural Science Edition);2002-02
4 LI Xin-qi 1,2, YUAN Long-ping 2 (1.Biological Science and Technology College, Central South University, Changsha, Hunan 410083, China; 2.China National Hybrid Rice Research and Development Center, Changsha, Hunan 410125, China);Molecular Strategies for Multiplication of Spontaneous and Artificial Genic Male Sterile Lines by Using Site-specific Recombination[J];Hybrid Rice;2004-05
5 Zhang Hongyu 1,Wang Xiuzhi 1,Zhao Xiuyun 2,Wang Xudong 1,Wu Xianjun 1* ( 1Rice research institute of Sichuan Agricultural University, Wenjiang Chengdu 611130; 2Institute of Genetics, Fudan University, Shanghai 200433);The Akvantage on Agrobacterium mediated Co-transformation Systems[J];Chinese Agricultural Science Bulletin;2004-02
6 SUN Jia-li,YAN Xiao-hong,WANG Li-jun,WEI Wen-hui (Department of Genomics and Molecular Biology,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Oil Crop Biology of the Ministry of Agriculture,Wuhan 430062);Progress in the Application Study of Cre/loxP Site-Specific Recombination System in Plants[J];Scientia Agricultura Sinica;2010-06
7 CHEN Ming1, 2, WANG Li-Xia1, PENG Xiang-Lei1, XU Hui-Jun2, LIN Zhong-Ping1*(1. State Key Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences,Peking University, Beijing 100871, China;2. Key Laboratory of Agriculture Ministry for Crop Genetics and Breeding, Institute of Crop Breeding and Cultivation, The Chinese Academy of Agricultural Sciences, Beijing 100081, China);Gene Expression Controlled by Heat-Inducible Site-Specific Recombination in Tobacco[J];植物学报(英文版);2003-12
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Ni Bin(College of Life Science Nanjing Agricultural University Nanjing Jiangsu 210095);Current Research Progress of Plant Marker free Transgenic Technology[J];Anhui Agricultural Science Bulletin;2007-16
2 He Yangpeng et al (Bureau of Forest of Qujiang Prefecture,Quzhou,Zhejiang 324022);Effect of Embryo Treatment and Hormone Concentration on Tall Fescue Callus Inducton[J];Journal of Anhui Agricultural Sciences;2003-03
3 HU Kai et al(College of Biological Science and Technology,Shenyang Agricultural University,Shenyang,Liaoning 110161);Analysis of the Cause of Contamination and Explant Sterilization in Plant Tissue Culture[J];Journal of Anhui Agricultural Sciences;2007-03
4 DUAN Yong-bo et al(College of Life Sciences,Guizhou University,Guizhou Key Laboratory of Agricultural Bioengineering,Guiyang,Guizhou 550025);Study on the Seed Germination and Seedling Chilling-Tolerance of Transgenic Rice with Isopentenyl Transferase(ipt)[J];Journal of Anhui Agricultural Sciences;2007-06
5 ZENG Jian-jun et al (College of Life Science,Jinggangshan College,Jian,Jiangxi 343009);Review and Prospect of Safety Research of Transgenic Crops[J];Journal of Anhui Agricultural Sciences;2007-22
6 CUI Guang rong (Anhui Technical Teachers College,Fengyang 233100);Methods and Characteristics of Plant Transgene and Gene Silence[J];Journal of Anhui Technical Teachers College;2003-01
7 Zheng Junbao Zhang Yuman Wang Xuerui (Hebei Forestry College,Baoding 071000)Pei Dong (Institute of Forestry,Chinese Academy of Forestry Science,Beijing 100091);Tissue Culture of Chimonanthus praecox[J];Journal of Beijing Forestry University;1995-S1
8 Han Liebao;Mu Xindai. Journal of Beijing Forestry University (2000)22(2)1~5[Ch,3 ref.] Institute of Turfgrass Science,Beijing For. Univ., 100083, P. R. China.;Disease of turfgrass and controlling in Lanzhou region.[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;2000-02
9 Zheng Yumei;Iiu Qinglin. Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100094, P. R. China.;Advances on in vitro micropropagation of woody ornamental plants.[J];Journal of Beijing Forestry University;2001-S2
10 Wang Cheng; Li Qing; Xin Yan. School of Landscape Architectur e, Beijing For. Univ.,100083, P.R.China.;Callus induction and plant regeneration from seeds of Festuca arundinacea[J];Journal of Beijing Forestry University;2004-01
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 YANG Yang et al(College of Life Sciences,Nanjing Xiaozhuang University,Nanjing,Jiangsu 211171);Research on the Poplar Transferred of AtNHX1 Gene with the cre/lox Expression Vector[J];Journal of Anhui Agricultural Sciences;2009-19
2 XU Yaying,WANG Yong,LUO Hong,CHEN Dian(College of Horticulture,Northeast Agricultural University,Harbin 150030,China);Integrating specific sequence into target gene using PCR method[J];Journal of Northeast Agricultural University;2011-04
3 LI Bo,YU Jing-juan,ZHAO Qian,ZHU Deng-yun,AO Guang-ming(State Key Laboratory for Agrobiotechnology,College of Biology,China Agricultural University,Beijing 100193);Transformation of the Gene pf40 in Maize[J];Scientia Agricultura Sinica;2009-09
4 Lei Yang;Li Yu;Wang Biao;Li Hongwei;Li Li;Wang Xifeng;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;;Construction of marker gene-free RNAi vector and genetic transformation of rice mediated resistance to Ricestripevirus[J];Plant Protection;2013-06
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