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《Acta Scicentiarum Naturalum Universitis Pekinesis》 2001-04
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Cre/loxP-mediated Deletion of Transgene in Transgenic Tobacco

ZHEN Wei WANG Yangming\ DING Wei\ CHEN Xi\ Hu Yuanlei\ LIN Zhongping (National Laboratory of Protein Engineering and Plant Genetic Engineering,Peking University,Beijing,100871)  
In the present study,we develop an efficient method to be used to examine recombination efficiency of transgenic plantlet.Two constructs,one containing Cre recombinase under the control of 35 S promoter (p35S/Cre) and the other containing a \%uidA\% gene driven by 35 S promoter flanked by loxP site (loxP/35S/GUS/loxP),were simultaneously introduced into tobacco by cotransformation method.The results show that Cre loxP system can precisely and efficiently direct deletion of transgene based on Gus assay,PCR analysis and sequence of recombination DNA fragment,but also find that incomplete deletion exist in partial plant.
【Fund】: 高等学校博士学科点专项科研基金资助项目
【CateGory Index】: Q784
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