Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Scientiarum Naturalium Universitatis Pekinensis》 2007-06
Add to Favorite Get Latest Update

Utilization of Sewage Sludge and Waste Molasses for Production of Bacillus thuringiensis Based Biopesticide

CHANG Ming 1)ZHOU Shungui 1),2)LU Na 1)NI Jinren 1),3)(1)Department of Environmental Engineering,Peking University,and the Key Laboratory of Water and Sediment Sciences,Ministry of Education,Beijing,100871; 2)Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control,Guangdong Institute of Eco-Environment and Soil Sciences,Guangzhou,510650  
Feasibility of combined fermentation of sewage sludge and waste molasses to Bacillus thuringiensis based biopesticide was studied.As a kind of useful supplemental carbon sources,waste molasses proved to be effective in promoting the growth and metabolism of Bacillus thuringiensis when the additive proportion was lower than 2.0%.Excessive molasses delayed the fermentation process and restrained the spores or crystals from maturing.The best combined medium was found to be sewage sludge(3% total solid)supplemented with 1.5 % molasses(w/w).In this medium,the highest viable cells count(VC)and viable spores count(VS)could rise up to 4.17×108CFU·mL-1and 3.60×108CFU·mL-1,respectively.Both crystal protein concentration and entomotoxicity of this medium were also superior to other combined media at the end of fermentation,with the highest crystal protein yield of 1.96mg·mL-1 and entomotoxicity of 788IU·μL-1,respectively.Moreover,significant correlation can be seen between the crystal protein yield and the entomotoxicity.Therefore,crystal protein yield can be used as a simple but reliable index for assessing the Bt fermentation process.
【Fund】: 广东省自然科学基金(5006694);; 广东省科技攻关(2005B10401008)资助项目
【CateGory Index】: TQ453
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved