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The Study of the Effect of Lanthanide Ions on the LDH Activity by Differential pulse Polarography

WANG Xunqing\ GAO Xiaoxia (College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871)  
Differential pulse polarography(DPP) was applied to study the effect of lanthanide ions on the LDH activity in the catalytical reaction of pyruvate+NADH+H +lactate+NAD +, by monitoring DPP reduction current of NAD +. In a buffer solution of 0.05 mol/L Tris HCl(pH7.5)+0.5 mmol/L pyruvate, NAD + exhibits a well defined differential pulse peak at -0.82 V. The peak current is proportional to NAD + concentration in the range 1 ̄28 μmol/L. In the assay mixture, low concentration La 3+ ions(5 μmol/L) slightly increase LDH activity. Other ions Ce 3+ ,Eu 3+ ,Dy 3+ and Yb 3+ (≥5 μmol/L) show inhibitory effects. The data of spectrophotometry and kinetics analysis are in agreement with DPP results.
【Fund】: 国家自然科学基金
【CateGory Index】: O657.14
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