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《ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS》 1999-02
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Purification and Enzyme Linked Immunosorbent Assay of Human Fetal Liver Metallothionein

ZHENG Junheng\ RU Gang\ RU Binggen (College of Life Sciences,Peking University,Beijing,100871)  
After human fetal liver was homogenized,centrifuged and precipitated with ethanol,two forms of metallothionein (MT 1,MT 2) were purified by gel filtration chromatography on Sephadex G 75,ion exchange chromatography on DEAE Sepharose Fast Flow.Their purity was verified by amino acid composition analysis.Using human MT 1 as antigen,the antiserum was produced in rabbit.Then enzyme linked immunosorbent assay (ELISA) was developed for the detection and quantitative analysis of human metallothionein,and the standard curves were supplied.
【Fund】: 国家科委九五攻关资助
【CateGory Index】: Q516
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【References】
Chinese Journal Full-text Database 10 Hits
1 LIU Lu;LIU Hui-ping;CHEN Ling-li;LIU Li;XIANG Qin;LI Ling;ZHU Ying-wu;College of TCM,Hunan University of Chinese Medicine;;Progress on detection method of metallothioneins[J];应用化工;2015-09
2 CHEN Jie;HAN Liang;LI Guoyue;WU Yi;LIN Huaqing;Institute of Materia Medica,Guangdong Pharmaceutical University;Zhanjiang Suoqi Biotechnology Company;;Progress on the separation and purification technology and function of metallothionein[J];广东药学院学报;2013-05
3 WANG Ming-he,CHEN Lei(Department of Clinical Laboratory,People′s Hospital of Pizhou City,Jiangsu 221300,China);The content changes of serum metallothionein in patients with chronic hepatitis B[J];检验医学与临床;2011-20
4 LIANG Peng,WU Xiao-ping,LIAO Ai-lin(College of food science and technology,Guangdong Ocean University,Zhanjiang 524025);Advancement of Metallothionein Detection Methods[J];中国食物与营养;2010-09
5 CAI Zhen-feng,REN Feng-lian(College of Chemistry and Chemical Engineering,Central South University,Changsha 410083,China);Research progress of metallothioneine[J];应用化工;2007-02
6 Zhang Yan1,2* Yang Chuanping2 1 Heilongjiang College of Education, Harbin, 150080; 2 Laboratory of Forest Genetics and Breeding, College of Forest, Northeast Forestry University, Harbin, 150040;Study Advances of Metallothionein[J];分子植物育种;2006-S1
7 JIANG Hua, ZHAO Yupei, CHEN Ge, et al. Department of General Surgery, Peking Union Medical College Hospital, Beijing 100730, P. R. China;Relationship between metallothionein level and radio-sensitivity of human pancreatic carcinoma cell lines[J];中华肝胆外科杂志;2006-11
8 WU Li-zhuan (College of Animal Science, Yangtze University, Jingzhou,Hubei 434025, China)LI Li-li (Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha,Hunan 410125,China)ZHANG Bin (College of Animal Science and Technology, Hunan Agricultural University, Changsha,Hunan 410128, China);The Preparation of the Mouse-antiswine Polyclonal Antiserum of Zn-Metallothionein(MT)[J];长江大学学报(自科版)农学卷;2006-02
9 WU Li-zhuan(College of Animal Science,Yangtze University,Jingzhou,Hubei 434025,China)LI Li-li(Institute of Subtropical Agriculture,Chinaese Academy of Sciences,Changsha,Hunan 410125,China)ZHANG Bin(College of Animal Science and Technology,Hunan Agricultural University,Changsha,Hunan 410128,China);The Preparation of the Mouse-antiswine Polyclonal Antiserum of Zn-Metallothionein(MT)[J];长江大学学报(自科版);2006-05
10 WU Li-zhuan~1,ZHANG Bin~1,LI Li-li~(2*)(1.College of Animal Science,Hunan Agricultural University,Changsha 410128,China;2.Institute of Subtropical Agriculture,The Chinese Academy of Sciences,Changsha 410125,China);The Preparation and Application of the Mouse-antiswine Polyclonal Antiserum of Zn-metallothionein(MT)[J];畜牧兽医学报;2006-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Li,YU Li-bo,LIU Ji-wen(Department of Occupational Health and Environmental Health,College of Public Health, Xinjiang Medical University,Urumqi 830011,China);The determination of the plant metallothione in part of plants in Xinjiang[J];新疆医科大学学报;2012-11
2 WU Jie1,3,WANG Hu1,QIU Ye1,et al(1.Department of Orthopedies,The Affiliated First Hospital of Shantou University Medical College,Shantou Guangdong 515041,China;2.Department of Meurdogy,The Certral Hospital of Shantou,Shantou Guangdong 515041,China;3.Department of Orthopedicss,The People's Livelihood Hospital of Chonan,Shantou Guangdong 515041,China);Diabetic peripheral neuropathy rats sufev metal proteir expresior meaning[J];吉林医学;2012-17
3 LIU Lu,LI Qiu,XUE Jin-hua,ZHOU Bin,WANG Yong-sheng(College of Public Health,University of South China,Hengyang 421001,China);Determination of metallothioneins by ultraviolet spectrophotometry with ciprofloxacin-Cu complex[J];应用化工;2012-03
4 Zhang Yanqiang1,2, An Lihui2,*, Zheng Binghui2, Lin Jin1, Chen Hao2, Zhao Xingru2 1.College of Chemistry & Material Science, Hebei Normal University, Shijiazhuang 050012, China 2.State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;Heavy Metal Levels and Metallothionein mRNA Expressions in Wild Crucian Carp (Carassius auratus auratus) from Hun River[J];生态毒理学报;2012-01
5 ZHENG Li-Ming1,2,ZHOU Fa-Lin1,YANG Qi-Bin 1,HUANG Jian-Hua1,QIU Li-Hua1,SU Tian-Feng1 and JIANG Shi-Gui1(1.South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China;2.Animal Sciences Institute,South China Agriculture University,Guangzhou 510642,China);MOLECULAR CLONING AND EXPRESSION ANALYSIS OF METALLOTHIONEIN FROM PENAEUS MONODON[J];水生生物学报;2011-06
6 FAN Lian-mei1,2,LIU Geng-sen 2,LIU Cheng-lian2,YUAN Yong-bing 2(1College of Life Science/Key Lab of Plant Biotechnology in Universities of Shandong,Qingdao 266109 2College of Landscape andHorticulture/Qingdao Key Lab of Modern Agriculture Qualiry and Safery Engineering,Qingdao Agricultural University,Qingdao 266109);Cloning and Bioinformatic Analysis of Metallothionein-like Protein Gene (MdFjMT2) from Apple(Malus domestica)[J];植物遗传资源学报;2011-05
7 ZHU Yao-min1,YUAN Zu-yi2,LIU Xiang1,JING Gui-xia1 1Department of Anesthesiology,2Department of Cardiology,First Affiliated Hospital,Xi'an Jiaotong University College of Medicine,Xi'an 710061,China;Effect of metallothionein on myocyte apoptosis and energy supply of isolated rabbit heart muscle during perfusion with ropivacaine[J];南方医科大学学报;2011-08
8 Jia Chaoli,Wu Tingting,Long Fangyi,Liu Zhimin(Department of Biochemistry and Molecular Biology,Institute of Molecular Medicine and Cancer,College of Basic Medical Sciences,Chongqing Medical University,Chongqing,400016,China);Hypoxia-induced metallothioneins promote epithelial-mesenchymal transition of human papillary thyroid carcinoma cells[J];第三军医大学学报;2011-07
9 LIANG Peng,WU Xiao-ping,LIAO Ai-lin(College of food science and technology,Guangdong Ocean University,Zhanjiang 524025);Advancement of Metallothionein Detection Methods[J];中国食物与营养;2010-09
10 Wang Xue Zhao Haifeng Lv Ming Li Hongyan Wu Wenjie Liu Ya Li Zhengqiang(Jilin University,Changchun 130021);The Purification Technology Metallothionein in Rat Liver[J];生物技术通报;2010-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 XUE Yanfeng;LUO Yihao;LIU Xiangjun;XIE Fenglian;JIANG Guobin;SUN Wancheng;Academy of Animal Science and Veterinary Medicine, Qinghai University;College of Agriculture and Animal Husbandry, Qinghai University;;Assessment of Heavy Metal Pollution by Expression of Yak Metallothionein Gene in Qinghai[J];食品科学;2015-18
2 LIU Lu;LIU Hui-ping;CHEN Ling-li;LIU Li;XIANG Qin;LI Ling;ZHU Ying-wu;College of TCM,Hunan University of Chinese Medicine;;Progress on detection method of metallothioneins[J];应用化工;2015-09
3 LUO Zheng-ming;JIA Lei-po;LIU Xiu-li;JIA Yan-qing;Department of Geography,Xinzhou Teachers University;Institute of zoology,Key Laboratory of Zoological Systematics and Evolution,Chinese Academy of science;;Research advances in toxic effect of cadmium on aquatic animals[J];食品工业科技;2015-15
4 TANG Xiu-li;WU Rui-qin;LIU Xin-li;School of Biological Engineering,Qilu University of Technology;The Institute of Disease Control and Prevention,Academy of Military Medical Sciences;;Effects of Metallothionein in Cyclophosphamide-Induced Immunosup Pression[J];齐鲁工业大学学报(自然科学版);2015-02
5 MAI Jie-Hui;YU Bo;ZHANG Qi-Zhong;Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes/Institute of Hydrobiology, Jinan University;;Prokaryotic Expression, Purification and Polyclonal Antibody Preparation of Crassostrea hongkongensis Metallothionein(MT)[J];农业生物技术学报;2015-08
6 Chen Zhong;Wei Li;Wu Sen;Zhang Ning;Zhu Xiaoming;Chen Mingyao;Department of Thoracic Surgery,People's Hospital of Henan Province;;Effects of metallothionein-3 expression on biological behaviors of A549 lung cancer cells[J];中华实验外科杂志;2014-10
7 MENG Hong-en;LIU Zhong-yuan;College of Life Science and Technology,Xinjiang University/Xinjiang Key Laboratory of Biological Resources and Genetic Engineering;;Research advances of plant metallothioneins[J];广东农业科学;2014-15
8 Hong-En Meng;Zhong-Yuan Liu;Xinjiang Key Labora-tory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University;;Metallothioneins and digestive system tumors[J];世界华人消化杂志;2014-19
9 Aiping HAO;College of Life Sciences and Technology,Mudanjiang Normal College;;Bioinformatics Analysis of Metallothionein in Betula phatyphylla[J];Agricultural Science & Technology;2013-12
10 KONG Fanna 1,2 , ZHOU Yang 1,2 , SUN Peipei 1,2 , LIU Limin 1,2 , MAO Yunxiang 1,2 1 College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China 2 Key Laboratory of Marine Biology Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China;Generation and analysis of expressed sequence tags from the salt-tolerant eelgrass species, Zostera marina[J];Acta Oceanologica Sinica;2013-08
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