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《Journal of Beijing University of Chemical Technology》 2002-05
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Integrated separation and purification of recombinant tumor necrosis factor from Escherichia coli

ZHU Ai hua 1 WANG Li 1 WANG Yun shan 2 SU Zhi guo 2 MA Run yu 1 (1. College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029; 2. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 10  
Recombinant human tumor necrosis factor alpha (rhTNF α) was released from Escherichia coli by bead mill. Further purification was tested by eight types of integrated precipitation with ammonium sulphate, utilizing the heat stability of rhTNF. The best result is that the ratio of rhTNF α to total protein is 57% and the recovery rate of rhTNF and its activity are 90 3% and 85 8%, respectively.
【Fund】: 国家杰出青年科学基金资助项目 (2 95 2 5 6 0 9) ;; 国家自然科学重点基金资助项目 (2 9736 180 )
【CateGory Index】: TQ464
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