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《Journal of Beijing University of Chemical Technology》 2002-05
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Integrated separation and purification of recombinant tumor necrosis factor from Escherichia coli

ZHU Ai hua 1 WANG Li 1 WANG Yun shan 2 SU Zhi guo 2 MA Run yu 1 (1. College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029; 2. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 10  
Recombinant human tumor necrosis factor alpha (rhTNF α) was released from Escherichia coli by bead mill. Further purification was tested by eight types of integrated precipitation with ammonium sulphate, utilizing the heat stability of rhTNF. The best result is that the ratio of rhTNF α to total protein is 57% and the recovery rate of rhTNF and its activity are 90 3% and 85 8%, respectively.
【Fund】: 国家杰出青年科学基金资助项目 (2 95 2 5 6 0 9) ;; 国家自然科学重点基金资助项目 (2 9736 180 )
【CateGory Index】: TQ464
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【Citations】
Chinese Journal Full-text Database 1 Hits
1  Xu Hao Gao Changshou Liu Hui Deng Fang Pan Hua Chen Changqing (Shanghai Research Center of Biotechnology,Chinese Academy of Sciences,Shanghai 200233);Downstream Technique Study of Human Tumor Necrosis Factor Alpha and its Mutant[J];CHINESE JOURNAL OF BIOTECHNOLOGY;1998-02
【Co-citations】
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1 YANG Ru yan, LI Min, CHEN Chang qing (Shanghai Research Center of Biotechnology,Chinese Academy of Sciences,Shanghai 200233);Determination and optimization of glycerol during the high density fermentation of genetic engineered bacteria[J];INDUSTRIAL MICROBIOLOGY;1999-01
2 ZHANG Chen, LIU Zhi-wei,GUO Yong (Department of Biology,Jiaying University, Meizhou 514015,China; College of Food Engineering and Biotechnology,South China University of Technology, Guangzhou 510640, China);Research Progress of Tumor Necrosis Factor and Its Genetic Engineering[J];Journal of Jiaying University;2002-06
3 ZHU Kai-Chun 1 ZHANG Fu-Chun 1 XIAO Chong2 HE Zhong-Huai2 JIN Hua-Li2 WANG Bin 1,2 (1.Key Laboratory for Bioresources and Bioengineering, College of Life Science and Technology, Xinjiang University, Urmuqi 830046, China; 2.State Key Laboratory for Agro-biotechnology, College of Biological Science, China Agricultural University, Beijing 100094, China);Large-Scale Plasmid DNA Preparation by Flow-Through Thermal Lysis[J];Journal of Agricultural Biotechnology;2006-01
4 WANG Fu qing 1, ZHU Rui 2 (1. China Biochemical & Pharmaceutical Industry Association 2. China Industrial Productivity Promotion Center of Biochemical Pharmacy, Beijing 100801, China);The latest advances in research of some important biochemical drugs[J];Chinese Journal of Biochemical Pharmaceutics;2003-02
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【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 Wang Guanghu\ \ Feng Xiaoli\ \ Xiu Zhilong \ \ Zhang Zhiqiang\ \ Su Zhiguo (Bioengineering Institute of Dalian University of Technology, Dalian 116012);Releasing of Tumor Necrosis Factor from Recombinant Escherichia Coli by Chemical Permeabilization and High Speed Bead Milling[J];JOURNAL OF CHEMICAL ENGINEERING OF CHINESE UNIVERSITIES;1996-03
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