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《Journal of Beijing University of Chemical Technology》 2004-04
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Repression of rHSA degradation during fermentation with transgenic yeast pichia pastoris

Sun Zhan sheng Chen Jin chun (College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China)  
One obvious problem for overexpression recombinant human serum albumin(rHSA) with the transgenic yeast pichia pastoris is degradation of the purpose proteins during fermentation. Minimization of degradation was therefore investigated for improving the yield of intact rHSA. The results showed that an addition of 0 5% yeast extract and 1% peptone (YP) to the medium was effective to decrease the degradation of the rHSA. Culture condition of high pH was also substantially reduced the degradation. However there was little effect by changing the temperature during the methanol feeding phase. By combining the optimal pH of 7 0 with the addition of YP(0 5%, 1 0%), the 58% intact rHSA was achieved.
【CateGory Index】: Q933
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