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《Journal of Beijing University of Chemical Technology》 2004-04
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Repression of rHSA degradation during fermentation with transgenic yeast pichia pastoris

Sun Zhan sheng Chen Jin chun (College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China)  
One obvious problem for overexpression recombinant human serum albumin(rHSA) with the transgenic yeast pichia pastoris is degradation of the purpose proteins during fermentation. Minimization of degradation was therefore investigated for improving the yield of intact rHSA. The results showed that an addition of 0 5% yeast extract and 1% peptone (YP) to the medium was effective to decrease the degradation of the rHSA. Culture condition of high pH was also substantially reduced the degradation. However there was little effect by changing the temperature during the methanol feeding phase. By combining the optimal pH of 7 0 with the addition of YP(0 5%, 1 0%), the 58% intact rHSA was achieved.
【CateGory Index】: Q933
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【References】
Chinese Journal Full-text Database 3 Hits
1 CHEN Si REN Rui CHEN Jin-chun(College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China);Selenium incorporation into recombinant human serum albumin in Pichia pastoris[J];Journal of Beijing University of Chemical Technology(Natural Science Edition);2006-05
2 LIN Jun-han(Fujian Vocational Technological College of Bio-Engineering,Fuzhou 350002,China);High Density Fermentation Control of Pichia pastoris[J];China Biotechnology;2009-05
3 XU Jue XU Chuan-lian DU Fang-yao(College of Life Science,Zhejiang Sci-Tech University,Hangzhou 310018,China);Optimization of Fermentation for ACE C-Domain from Pichia pastoris[J];China Biotechnology;2010-04
【Citations】
Chinese Journal Full-text Database 2 Hits
1 GUO Mei\|Jin WU Kan\|Hua HANG Hai\|Feng CHU Ju ZHUANG Ying\|Ping ZHANG Si\|Liang (State Key Lab of Bioreactor Engineering,East China University of Science & Technology,Shanghai 200237) 1 (Research and Development lenter for Biotechnology,Jiang Xi Ag;STUDIES ON CONDITIONS OF CELL HIGH DENSITY CULTIVATION FOR GENETICALLY ENGINEERED METHYLOTROPHIC PICHIA PASTORIS[J];Microbiology;2001-03
2 XIE Jing li 1, ZHANG Li 1, YE Qin 1, ZHOU Qing wei 2, XIN Li 2, DU Peng 2, GAN Ren bao 2 (1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; 2. Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China);Methanol Feeding Control in High-Density Cultivation of Mut~s Recombinant Pichia pastoris[J];Journal of Wuxi University of Light Industry;2003-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 SHAN Jun et al(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou,Gansu 730000);Study on the Metabolic Process of Organic Acids in Beer[J];Journal of Anhui Agricultural Sciences;2008-15
2 ZHANG yu et al(College of Biological Science and Engineering,South China University of Technology,Guangzhou,Guangdong 510006);Secreted Gene Expression and Fermentation Optimization of Rhizomucor miehei Lipase in Pichia pastoris[J];Journal of Anhui Agricultural Sciences;2010-35
3 Tan Tianwei;Qi Yizheng;Luo Hui;He Xianfeng(Department Chemical Engineering Beijing University of Chemical Technology, Beijing, 100029);Kinetic Models of the Fermentation for Ergosterol with Yeast[J];JOURNAL OF BEIJING UNIVERSITY OF CHEMICAL TECHNOLOGY;1996-01
4 CHEN Xuan1, TU You-ying1, TONG Qi-qing1, CHEN Li-yan1, LIN Zhi2 (1. Tea Department, Zhejiang University, Hangzhou 310029, China; 2. Key Lab of Tea Chemical Engineering of Agricultural Ministry, Hangzhou 310008, China );Study on the Metabolic Kinetics of Active Components in Green Tea Extract During the Fermentation By Yeast[J];Journal of Tea Science;2002-01
5 Zhou Gnangqi;Xu sukui;Yan Fu;ALCOHOL PRODUCTION BY IMMOBILIZED YEAST[J];;1994-01
6 Zhang Xiaoyun Wei Yineng ( Department of Biology, Guangxi Normal University, 541004,Guilin,PRC);THE ELECTROPHORESIS OF MDH AND SOD IN DTMORPHISM OF CANDIDA TRIPILIS[J];JOURNAL OF GUANGXI NORMAL UNIVERSITY(NATURAL SCIENCE);1998-04
7 Song Yiling, Wang jin, Wei Shuzhong(Department of Food Engineering, Shandong Light Industry Institute, Jinan 250100);Effect of Fluoride on Alcohol Production[J];Industrial Microbiology;1997-01
8 WU Xiao-jie,HE Ning,SUN Dao-hua,LIN Xue-ping,CHEN Cui-xue, DENG Xu,LI Qing-biao (Department of Chemical and Biochemical Engineering,Key Lab for Chemical Biology of Fujian Province,Xiamen University,Xiamen 361005);Effects of metal ions on production of bioflocculant REA-11[J];Industrial Microbiology;2006-02
9 ZHU Zhi-Gang~1,CHU Ju~1,HU Xiao-Qing~1,ZHUANG Ying-Ping~1,WANG Yong-Hong~1,ZHANG Si-Liang~(1*),YUAN Zhong-Yi~2(1.State Key Lab of Bioreactor Engineering,National Engineering Center for Biotechnology(Shanghai),East China University of Science and Technology,Shanghai 200237;2.Institute of Biochemistry and Cell Biology,Shanghai Institutes for Life Science,the Chinese Academy of Sciences,Shanghai 200031);Optimization on culture conditions for producing S-adenosyl-L-methionine by recombinant Pichia pastoris[J];Industrial Microbiology;2006-03
10 WANG Ying,HE Ning,LI Qing-biao,DENG Xu,LU Ying-hua(Department of Chemical and Biochemical Engineering,Xiamen University,Xiamen 361005);Optimization of high cell density cultivation conditions of Saccharomyces cerevisiae[J];Industrial Microbiology;2007-01
China Proceedings of conference Full-text Database 1 Hits
1 WANG Dong~1 LIU Ying-cai~1 LI Wen-ke~2 (1.Institute of Material Science & Engineering,Ocean University of China,Qingdao 266100,China;2.School of Materials Science and Engineering,University of Science and Technology Beijing,Beijing 100083,China);Preparation of ZnO nanoparticles by bionic method and characterization[A];[C];2007
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YIN Shu-qin et al(College of Life Sciences,Shanxi Agricultural University,Taigu,Shanxi 030801);Research on High Cell Density Culture of Genetic Engineering Bacteria[J];Journal of Anhui Agricultural Sciences;2007-11
2 WU Ri-na et al (College of Animal Science and Techonology,Yangzhou University,Yangzhou,Jiangsu 225009);Effects of Phytase on Growth Performance and Digestibility of Piglets[J];Journal of Anhui Agricultural Sciences;2008-19
3 LU Qi-xiang, ZHAO Shu-jinSouth China University of Technology, Guangzhou 510641,China;Optimization of Heterologous Protein Expression in Pichia pastoris[J];Amino Acids & Biotic Resources;2005-04
4 BAO Ming~1,ZHONG Da-lian~2,XU Fa-zhi~1,YU Wei-yi~(1*),LI Jin-nian~1(1.College of Animal Science and Fisheries, Anhui Agricultural University, Hefei, 230036;2.College of Life Science, University of Science and Technology of China);Cloning and Expression of Chicken Interferon-gamma Gene[J];Journal of Anhui Agricultural University;2004-01
5 DENG Min 1, HE Jian guo 1, ZUO Tao 1, WENG Shao ping 1 ZENG Kang 1, LU Ling 1,ZHOU Song yu 1,LONG Qing xin 2 (1.School of Life Sciences, Zhongshan University, Guangzhou 510275, China; 2.State Key Laboratory of Biocontrol, Zhongshan Univers;Infectious Spleen and Kidney Necrosis Virus (ISKNV) from Siniperca Chuatsi : Development of a PCR Detection Method and the New Evidence of Iridovirus[J];Chinese Journal of Virology;2000-04
6 DENG Min 1,HE Jian guo 1,WENG Shao ping 1,ZENG Zheng 1, LONG Qing xin 2 (1 School of Life Sciences,Zhongshan University,Guangzhou 510275,China; 2 State Key Laboratory of Biocontrol,Zhongshan University,Guangzhou 510275,China);Molecular Cloning and Physical Mapping of the Genome of Infectious Spleen and Kidney Necrosis Virus(ISKNV) from Mandarinfish,Siniperca chuatsi(Basilewsky)[J];Chinese Journal of Virology;2001-03
7 HE Hua-hong,DENG Min,HE Jian-guo,WENG Shao-ping (School of Life Science,Zhongshan University,Guangzhou 510275,China);Identification and Characterization of the Infectious Spleen and Kidney Necrosis Virus Cytosine DNA Methyltransferase Gene[J];Chinese Journal of Virology;2001-04
8 SUN Mei-yu,QIN Li-ting,GAO Yu-long,QI Xiao-le,GAO Hong-lei,WANG Yong-qiang,WANG Xiao-mei(Division of Avian Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy Agricultural Sciences,Harbin 150001,China);Expression and Identification of σC Gene of Avian Reovirus by Baculovirus Expression System[J];Chinese Journal of Virology;2011-04
9 LI Ming WANG Xiao Ming SHENG He Lin (Department of Biology,East China Normal University,Shanghai 200062, China);MITOCHONDRIAL DNA DIVERGENCE AND PHYLOGENY OF FOUR SPECIES OF DEER OF THE GENUS CERVUS[J];ACTA ZOOLOGICA SINICA;1999-01
10 LIU Yan-li 1 LIU Yong-dong 2 WANG Yan-hui 1 WANG Yun-shan 2 SU Zhi-guo 2 MA Run-yu 1 (1. The Key Laboratory of Science and Technology of Controllable Chemical Reactions, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029; 2. The State Key Lab of Biochemical Engineering, the Institute of Process Engineering of the Chinese Academy of Sciences, Beijing 100080, China);High density culture conditions of recombinant human serum album from Pichia pastoris[J];Journal of Beijing University of Chemical Technology;2003-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 YI Bo-wen et al(School of Bioscience and Bioengineering,South China University of Technology,Guangzhou,Guangdong 510006);Research Progress in Chemostat Culture of Recombinant Pichia pastoris[J];Journal of Anhui Agricultural Sciences;2010-27
2 ZHU hua1,YU Hong-wei1,GUO Run-fang1,JIA Ying-min2(1.College of Food Science and Technology,Agricutural University of Hebei,Baoding 071001,China; 2.College of Bioscience and Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China);Optimization of the fermentation conditions of the engineering strain producing glucose oxidase[J];Journal of Agricultural University of Hebei;2012-03
3 CUI Jing-ai;FANG Dan-dan;CHEN Xiao-ping;College of Food Science and Engineering, Jilin Agricultural University;;Study on Shake-flask Culture Conditions of Spinigerin α Antibacterial Peptides[J];Journal of Jilin Agricultural University;2013-06
4 Cui Yanyan1,2,Yi Guohui1,Tu Fazhi1,Zhang Tianyuan3,Luo Jinxian3,Zhang Ailian1 1 Key Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;2 College of Agronomy,Hainan University,Danzhou,Hainan 571737,China;3 The Key Laboratory of Gene Engineering of Ministry of Education and Department of Biochemistry,Sun Yat-Sen University,Guangzhou,Guangdong 510275;Constitutive Expression of Cellobiohydrolase Ⅱ in P.pastoris by High-density Cell Culture[J];Chinese Journal of Tropical Crops;2010-08
5 Xiaofeng Wang,Yongchuan Sun,Xuguang Shen,Feng Ke,Li Xu,Yun Liu,and Yunjun Yan Key Laboratory of Molecular Bio-physics,College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China;Expression of Vitreoscilla hemoglobin improves recombinant lipase production in Pichia pastoris[J];Chinese Journal of Biotechnology;2011-12
6 Jiao Long1,Yu Hong-wei1,Guo Run-fang1,Bai Yu1,Jia Ying-min21(College of Food Science and Technology,Agricultural University of Hebei,Baoding 071001,China) 2(College of Bioscience and engineering,Science and Technology University of Hebei,Shijiazhuang 050018,China);Study on the Fermentation for Fibrinolytic Enzyme from Pichia pastoris pk53[J];Food and Fermentation Industries;2011-03
7 SA Rong-bo1,SHI Gui-yang2,TANG Yu-jing1 (1. Bioscience Department,Taishan Medical University,Taian 271016,China; 2. School of Biotechnology,Jiangnan University,Wuxi 214036,China);Influence of different feeding strategies on high density culture of recombinant pichia pastoris[J];Science and Technology of Food Industry;2010-11
8 DAI Yi-xing,WANG Hong-xin,LV Wen-ping*,SUN Jun-tao,QIN Xiao-juan(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China);Expression and characteristic of β-Glucanase in Escherichia coli induced by lactose[J];Science and Technology of Food Industry;2011-09
9 ZHUANG Ling-xi1,DUAN Ran1,CHEN Long-jun2,LING Xue-ping1,LU Ying-hua1,(1.College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China;2.Guangzhou Sugarcane Industry Research Institute,Guangzhou 510316,China);Expression of α-1,6-dextranase from Penicillium minioluteum in P.pastoris[J];Science and Technology of Food Industry;2011-11
10 YANG Sui-shan1,2,YI Guo-hui1,TU Fa-zhi1,ZHANG Tian-yuan3,LUO Jin-xian3,ZHANG Ai-lian1(1.Key Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;2.College of Life Science and Agriculture,Hainan University,Haikou 570228,China;3.Key Laboratory of Gene Engineering of Ministry of Education and Department of Biochemistry,Sun Yat-Sen University,Guangzhou 510275,China);Inducible Expresstion of CellobiohydrolaseⅡ by AOXI Promoter in P.pastoris Using High-density Cell Culture[J];Biotechnology;2010-06
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 XIN Li, ZHANG Li 1, XU Ren, ZHANG Qian, YE Qin 1, LI Zai Ping, GAN Ren Bao * ( Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China; 1 Stat;Expression of Human Angiostatin in Pichia pastoris and the Detection of Its Anti-angiogenic Activity[J];Acta Biochimica Et Biophysica Sinica;2001-03
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