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Biohydrogen produced through mixture anaerobic biological fermentation

ZHAO Yu-shan TENG Xiao-feng ZHANG Peng TAN Tian-wei (College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China)  
The substrate containing glucose was treated with natural anaerobic microorganism bacterium from sullage. The biohydrogen was produced through anaerobic hydrogen fermentation. Under the experimental conditions, the maximum hydrogen productivity was 2.1 mol/mol (H_2/glucose) under the conditions of optimal initial pH value of 5.5. The optimal concentration of glucose was 5~30 g/L. The optimal substrates were glucose, pentose and starch. The hydrogen concentration in the biogas reached 97% without methanogenesis in the gaseous product, after it passed through 5% sodium hydroxide solution. It was found that the fermentation type was a butyric acid type fermentation.
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