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《Journal of Beijing University of Chemical Technology》 2005-06
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Extraction of RNA from waste Penicillium biomass

LI Shi-kun TAN Tian-wei(College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China)  
The conditions of extraction and purification of RNA from waste Penicillium biomass were studied.A salt and alkali method was used for cell disruption,the pH value of extraction solution was adjusted to 4.6 to precipitate part of protein,and the supernatant after fractional precipitation was treated with neutral protease AS1.398.The amount of AS1.398 neutral protease,reaction temperature, initial pH value and the hydrolytic time were explored.Then the hydrolysate was treated with ultrafiltration membrane(MWCO is 6.0×10~4),and the purity and the yield of RNA was 72.1% and 0.42%,respectively.
【Fund】: 国家863项目(2002AA601280);; 国家自然科学基金项目(20136020 50373003)
【CateGory Index】: TQ464.7
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1 He Guoqing, Xiong Haoping, Ruan Hui, Chen Qihe, Xu Ying(College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China);Optimization of condition for extracting polyphenols from Humulus lupulus[J];Transactions of The Chinese Society of Agricultural Engineering;2005-04
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