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Response surface analysis of lipase production by Penicillium camembertii Thom

ZHANG Da-hao TAN Tian-wei WANG Bing-wu (College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China)  
Optimum medium composition in lipase production via Penicillium camembertii Thom PG-3 fermentation was investigated using Response Surface Methodology(RSM).The impact of initial medium constituents on lipase production was studied by Fractional Factorial Design(FFD),revealing that both ammonium secondary phosphate and fat-free soybean meal have a substantial influence on lipase production.In our work,the greatest response area was estimated by the design of steepest ascent approach,and the optimum concentrations of ammonium secondary phosphate and fat-free soybean meal were confirmed via Central Composite Design(CCD).The quadratic model established by regression analysis facilitated the prediction of the optimum medium composition for the fermentation process,and results of the experiment were in agreement with our proposed model.An increase in lipolytic activity concentration with ultimate value 43% was observed after the optimization carried out according to our RSM design.
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