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Immobilization of fusarium oxysporum BU-11 cells by alginate composite for optical resolution of racemic DL-pantolactone

WANG Xue-mei YU Ming-rui TAN Tian-wei(College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China)  
A processing of entrapping Fusarium oxysporum BU-Ⅱ in calcium alginate,alginate-PAM and alginate-PVA composite gel beads was investigated.The immobilized cells were applied in the optical resolution of racemic DL-pantolactone producing D-pantoic acid.It is shown that the alginate immobilized cells have good diffusivity and strength.Compared with the free cells,the enzyme activity of the immobilized cells reached to 80%.Further more,the alginate immobilized cells showed a good temperature and operation stability.A batched hydrolysis by the immobilized cells was carried out in a 200?g/L substrate solution every 6?hours in a 3?L stirred tank reactor.After 20 batches of the hydrolysis reaction,the enzyme activity reached 80% of the original activity.It is shown that the addition of PVA enhances the strength of the immobilized cells,with little change in the enzyme activity,and while the addition of PAM induces the enhancement of not only the strength but also the enzyme activity of the immobilized cells.
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