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《Journal of Beijing University of Chemical Technology(Natural Science Edition)》 2009-01
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The application of tobacco and MPTA during lycopene production by Blakeslea trispora

XIU Yu YUAN QiPeng(College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China)  
The efficacy and optimization of the application of two inhibitors,tobacco and bleaching herbicide 2-(4-methylphenoxy)-triethylamine(MPTA),on lycopene productivity by Blakeslea trispora were investigated.The greatest yield of lycopene(170 mg/L)was observed when nicotine in the form of tobacco was added at 15 g/L at 48 hours old mated cultures,however,this addition did not inhibit(-carotene synthesis to the same extent as MPTA's,but resulted in 53% increase in overall carotenoid synthesis.Lycopene biosynthesis was strongly stimulated(205 mg/L)at the expanse of(-carotene when 2.2 mmol/L MPTA and 10 g/L tobacco added together at 48 hours following inoculation.Because of the low quantity but great efficiency,this compound inhibitor of tobacco and MPTA will become a potential choice of inhibiting lycopene cyclase in Blakeslea trispora.
【Fund】: 国家自然科学基金(20376007)
【CateGory Index】: TQ929
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