Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of University of Science and Technology Beijing》 2005-04
Add to Favorite Get Latest Update

Isolation and heterotrophic culture of Chlorella sp

YAN Hai, ZHOU Jie, HE Hongsheng, WEI Yuxia, SUN Jianxin Applied Science School, University of Science and Technology Beijing, Beijing 100083, China Guangdong Lvbaiduo Biotechnology Company, Zhanjiang 524017, China  
A strain of microalga that has a strong ability in the utilization of organic compounds was isolated from Qinghe River in Beijing and identified as Chlorella sp. The effects of nitrogen compounds and carbon-to-nitrogen mass ratio on the growth of this isolated Chlorella sp were investigated in batch cultures. Using glucose as the sole carbon source, both potassium nitrate and urea could be used as the sole nitrogen sources to support the continuous growth of Chlorella sp, respectively. When ammonium chloride was used as the sole nitrogen source, the growth of Chlorella sp could be apparently inhibited by the rapid decrease in pH of culture solution. When glucose and potassium nitrate were used as the sole carbon and nitrogen sources, respectively, the growth of Chlorella sp increased with the increase of carbon-to-nitrogen mass ratio in range from 5 to 20, and the maximum OD680nm of 73.8 was obtained.
【Fund】: 国家自然科学基金(N0.30270277 No.20377047) 北京科技大学422高级引进人才及校重点基金(No.20050604190)资助项目
【CateGory Index】: Q943.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 WANG Suqin1,2), LI Yawen3), YAN Hai1), YANG Shuai2), LIN Hai2)1) Applied Science School, University of Science and Technology Beijing, Beijing 100083, China2) Civil and Environmental Engineering School, University of Science and Technology Beijing, Beijing 100083, China3) Department of Biological Technology, Beijing University of Aeronautics and Astronautics, Beijing 100083, China;Heterotrophic culture of Chlorella sp USTB01 and production of lutein[J];Journal of University of Science and Technology Beijing;2007-08
2 YIN Chun-hua1),XU Pei1),HE Huan-ju1),JIA Xuan1),WANG Hai-ou1),JIANG Ze-yi2),WANG Ge2),ZHANG Xin-xin2),YAN Hai1) 1) School of Chemical and Biological Engineering,University of Science and Technology Beijing,Beijing 100083,China 2) School of Mechanical Engineering,University of Science and Technology Beijing,Beijing 100083,China;Conversion of Chlorella oil to biodiesel catalyzed by bronsted acid ionic liquid[J];Journal of University of Science and Technology Beijing;2011-11
3 ZHU Bi-yin,CHEN Yan,LIN Yu-shuang,HONG Wen-rong*(College of Biological Science and Technology,Fuzhou University,Fuzhou 350108,China);The separation of C.vulgaris and the study of algae biomass[J];Strait Pharmaceutical Journal;2008-11
4 XU Qian-qian1,JING Jian-ke1,2,LIU Shuo1,2,ZHANG Bin1,2,YAN Hai1(1.Department of Biological Science and Technology,School of Applied Science,Beijing University of Science and Technology,Beijing 100083,China;2.Department of Environmental Engineering,School of Civil & Enviromental Engineering,Beijing University of Science and Technology,Beijing 100083,China);Study on the Production of Eicosapentaenoicacid from Chlorella USTB-01[J];Chemistry & Bioengineering;2008-08
5 Jia Xuan1 Yan Hai1 Xiao Baoqing1 Ma Ying2(1.Department of Biological Science and Technology,School of Applied Science,Beijing University of Science and Technology,Beijing 100083,China;2.Baotou Research Institute of Rare Earths,Baotou 014030,China);Removal of total nitrogen from wastewater discharged from a chemical fertilizer plant by Chlorella protothecoides USTB-01[J];Chinese Journal of Environmental Engineering;2010-04
6 LI Ao-bo,WANG Pin,ZHANG Zheng-jie,JIU Wei-jing,LI Jin-sui College of Chemistry and Environmental Engineering,Beijing Technology and Business University,Beijing 100048,China;Study on Isolation and Identification of a Strain Heterotrophic Chlorella and its Growth Characteristics[J];Journal of Environmental Engineering Technology;2011-02
7 WANG Xiao-dan1,2,LI Sheng1,2,QIU Shu-yi1,2,3,HE Min3,HE Li3 (1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy,Guiyang 550003,China;2. Department of Chemical Engineering,Guizhou University,Guiyang 550003,China;3. Guizhou Province Base of Material Innovation,Guiyang 550000,China);Preparation of a Novel Biocide Using Poly Quaternary Ammonium Salt Grafted Nylon[J];Fine Chemical Intermediates;2007-02
8 WANG Su-qin1,2, YAN Hai1, ZHANG Bin1,2, LU Le1, LIN Hai21. Department of Biological Science and Technology, University of Science and Technology Beijing, Beijing 100083, China2. Department of Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China;Effects of Various Nitrogen Sources and Phytohormones on Growth and Content of Lutein in Chlorella sp USTB01[J];Science & Technology Review;2005-12
9 ZHANG Xue-Cheng1,SHI Yan-Xia1,MENG Zhen2(1.College of Marine Life Science,Ocean University of China,Qingdao 266003,China;2.Key Laboratory for Sustainable Utilization of Marine Fisheries Resource,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Science,Qingdao 266071,China);The UV-Irradiation of Chlorella. vulgaris Beijerinck and Screening of Productive Strain[J];Periodical of Ocean University of China;2007-05
10 KONG Wei-bao1,2,LI Long-nan1,ZHANG Ji1,XIA Chun-gu2,*(1.College of Life Science,Northwest Normal University,Lanzhou 730070,China;2.State Key Laboratory for Oxo Synthesis and Selective Oxidation,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China);Healthcare Functions and Applications in Food Industry of Chlorella[J];Food Science;2010-09
【Citations】
Chinese Journal Full-text Database 4 Hits
1 ZHANG Li-jun, YANG Ru-de, XIAO Heng ( The Food and Biotechnology Institute of South China University of Technology, Guangzhou 510640, China );The hetertrophic culture of Chlorella and optimization of growth condition[J];Guihaia;2001-04
2 Yu Ruoqian, Liu Xueming, Liang Shizhong and Chen Zijian College of Food Engineering & Biotechnology, South China University of Technology, Guangzhou 510640 Sericulture Research Institute of Guangdong Academy of Agricultural Sciences, Guangzhou 510640 Jian;Study on the Heterotrophic Growth Characteristics of Chlorella vulgaris[J];Marine Science Bulleten;2000-03
3 ZHANG Haibin; SUN Shichun; MAI Kangsen; LIANG Ying (Aquaculture Research Lab, Ocean University of Qingdao, Qingdao, 266003, China);ADVANCES IN THE STUDIES ON HETEROTROPHIC CULTURE OF MICROALGAE[J];TRANSACTION OF OCEANOLOGY AND LIMNOLOGY;2000-03
4 LIU Xueming, YU Ruoqian, LIANG Shizhong(The College of Food Bioengineering and Biotechnology, South China University of Technology, Guangzhou 510641);THE RELATIONSHIP BETWEEN OPTICAL DENSITY AND DRY WEIGHT OF CHLORELLA VULGARIS IN BATCH HETEROTROPHIC CULTURE.[J];MICROBIOLOGY;1999-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Shao-hua et al (Life Science Department of Nanjing Xiaozhuang University, Nanjing, Jiangsu 210017);Effect of Selenium (Se) on the Growth and Quality of Chlorella vulgaris[J];Journal of Anhui Agricultural Sciences;2006-09
2 JIANG Hong-xia et al (College of Life Sciences,Henan Normal University,Xinxiang,Henan 453007);Studies on Antimicrobial Activities against Three Plant Pathogen by Using Eleven Microalgae Extracts[J];Journal of Anhui Agricultural Sciences;2008-10
3 LV Xu-yang et al(Department of Life Science,Nanjing Xiaozhuang College,Nanjing,Jiangsu 211171);Methodological Research on Measuring Chlorella Quantity by Spectrophotometry[J];Journal of Anhui Agricultural Sciences;2009-23
4 JI Xiang et al(Institute of Bioengineering and Technology,Inner Mongolia University of Science and Technology,Baotou,Inner Mongolia 014010);Culture of Chlorella spp and Optimization of Growth Condition[J];Journal of Anhui Agricultural Sciences;2009-34
5 WU Xiao-dan et al (College of Life Science and Food Engineering,Nanchang University,Nanchang,Jiangxi 330031);Application of Biotechnology to Construct A Sustainable Biodiesel Production System from Microalgae[J];Journal of Anhui Agricultural Sciences;2010-13
6 CAI Jin-zhong et al(Laboratory of Food and Drug Safety Test,Xiamen Huaxia Vocational College,Xiamen,Fujian 361024);Research on the Fluorescence Characteristics of Synechococcus sp.and Salt Algae[J];Journal of Anhui Agricultural Sciences;2010-17
7 ZHANG Wen-ting et al(College of Life Sciences,Jiangxi Normal University,Nanchang,Jiangxi 330022);Light Attenuation of Synechocystis sp. PCC 6803 in Photo-bioreactor and Its Growth Dynamics Research[J];Journal of Anhui Agricultural Sciences;2010-19
8 LIU Yun-he et al(Ocean College of Huaihai Institute of Technology,Lianyungang,Jiangsu 222005);Study on Accumulation Condition of Fatty Acid in Isochrysis galbana OA-3011[J];Journal of Anhui Agricultural Sciences;2011-04
9 LU Xin-sheng et al(Department of Chemistry and Life Sciences,Gansu Normal University for Nationalities,Hezuo,Gansu 747000);Determination of Pb Concentrations from Plant Leaves by Extraction-Luminosity Method[J];Journal of Anhui Agricultural Sciences;2011-13
10 SHI Jian-le et al(College of Environment,Nanjing University of Technology,Nanjing,Jiangsu 210009);Study on the Improvement of Chlorophyll a Measuring Method in the Marine Diatom[J];Journal of Anhui Agricultural Sciences;2011-23
China Proceedings of conference Full-text Database 4 Hits
1 TONG Mu,ZHOU Zhi-Gang (Key Open Laboratory of Utilization of Aquatic Germplasm Resources of Minisrry of Agriculture of Shanghai Ocean University,E-Research Institute of Aquaculture of Shanghai Universities,Shanghai,201306,China);A New Generation of Bio-diesel Raw Material--Microalgae[A];[C];2009
2 Han Xu, Xiaoling Miao, Qingyu Wu(Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, P. R.China);HIGH QUALITY BIODIESEL PRODUCTION FROM HETEROTROPHIC GROWTH OF CHLORELLA PROTOTHECOIDES IN FERMENTERS BY USING STARCH HYDROLYSATE AS ORGANIC CARBON[A];[C];2005
3 Zhao Zengchun,Shen Liangfu (The Monitoring Center of the National Ocean Environment.Dalian 116023);THE SPECIES DIVERSITY OF DIATIOM-PLANKTON AND THE FACTORS AFFECTING IT IN DALIAN AND DAYAO BAYS[A];[C];1994
4 WU Lingling and LU Jianjian (State Key Laboratory of Estuarine and Coastal Research,East China Normal University,Shanghai 200062);Biodiversity and Ecosystem Services Value of Wetlands in the Yangtze River Estuary[A];[C];2002
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Yan Meijiao1 Wang Yindong2 Hu Xianjiang2 (1 Animal Science and Technology College of Yangzhou University,YangZhou,JiangSu,225009;2 Fishery Institute of Anhui Academy of Agricultural Sciences,HeFei,AnHui,230051);Impact of Illumination on Chlorella vulgaris and Scenedesmus Obliquusz Growth Rate and Chlorophyll Contents[J];Anhui Agricultural Science Bulletin;2007-23
2 LIU Shao-hua et al (Life Science Department of Nanjing Xiaozhuang University, Nanjing, Jiangsu 210017);Effect of Selenium (Se) on the Growth and Quality of Chlorella vulgaris[J];Journal of Anhui Agricultural Sciences;2006-09
3 WANG Li-bao et al (Institute of Marine Fisheries,Nantong,Jiangsu 226007);Heterotrophic Nitrification and its Application in Aquiculture[J];Journal of Anhui Agricultural Sciences;2006-22
4 SONG Guan-ling(Department of Life Science,Shandong University of Technology,Zibo,Shandong 255049);Application of the Bioremediation Technology in Eutrophic Waterbody Treatment[J];Journal of Anhui Agricultural Sciences;2007-27
5 LV Xu-yang et al(Department of Life Science,Nanjing Xiaozhuang College,Nanjing,Jiangsu 211171);Methodological Research on Measuring Chlorella Quantity by Spectrophotometry[J];Journal of Anhui Agricultural Sciences;2009-23
6 SONG Zeng-fu et al(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306);Isolation,Identification and Selection of Preventable Drugs of One Strain of Pathogen from Rotten-mouth-diseases in Rana[J];Journal of Anhui Agricultural Sciences;2009-34
7 YUAN Tian-kun et al(Hainan Tropical Key Laboratory of Aquatic Biotechnology,Ministry of Education Key Laboratory for Tropical Biological Resources,Maritime Academy,Hainan University,Haikou,Hainan 570228);Effect of Two Kinds of Culture Medium on Growth of Chlorella vulgaris[J];Journal of Anhui Agricultural Sciences;2010-16
8 SUN Chuan-fan et al(China Rural Technology Development Center,Ministry of Science and Technology of P.R.China,Beijing 100045);The Effects of Environmental Factors on the Lipid Accumulation in Microalgae[J];Journal of Anhui Agricultural Sciences;2011-19
9 FANG Li-chao, WANG Bin, ZHANG Jing-liang, LIU Rui-lan, YUAN Jing, WEI Hong (The laboratory animal center of Third Military Medical University, Chongqing 400038, China);Safety Evaluation of A Potential Probiotic Rhodopseudomonas palustris 2c[J];Amino Acids & Biotic Resources;2005-02
10 ZHOU Hua-wei~1, LIN Wei-tie~1, CHEN Tao~2(1.School of Bioscience and Bioengineering, South China University of Technology , Guangzhou510641, China ; 2. South China Sea Insititute of Oceanology , Chinese Academy of Science , Guangzhou 510301, China);The Heterotrophy and Applications of Chlorella[J];Amino Acids & Biotic Resources;2005-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Yue-qun et al(Nantong Agricultural Vocational and Technical College,Nantong,Jiangsu 226007);Effects of Ultraviolet Radiation on the Protein Contents of Three Marine Micro-algae Species[J];Journal of Anhui Agricultural Sciences;2009-20
2 LV Xu-yang et al(Department of Life Science,Nanjing Xiaozhuang College,Nanjing,Jiangsu 211171);Methodological Research on Measuring Chlorella Quantity by Spectrophotometry[J];Journal of Anhui Agricultural Sciences;2009-23
3 WANG Suqin1,2), LI Yawen3), YAN Hai1), YANG Shuai2), LIN Hai2)1) Applied Science School, University of Science and Technology Beijing, Beijing 100083, China2) Civil and Environmental Engineering School, University of Science and Technology Beijing, Beijing 100083, China3) Department of Biological Technology, Beijing University of Aeronautics and Astronautics, Beijing 100083, China;Heterotrophic culture of Chlorella sp USTB01 and production of lutein[J];Journal of University of Science and Technology Beijing;2007-08
4 YIN Chun-hua1),XU Pei1),HE Huan-ju1),JIA Xuan1),WANG Hai-ou1),JIANG Ze-yi2),WANG Ge2),ZHANG Xin-xin2),YAN Hai1) 1) School of Chemical and Biological Engineering,University of Science and Technology Beijing,Beijing 100083,China 2) School of Mechanical Engineering,University of Science and Technology Beijing,Beijing 100083,China;Conversion of Chlorella oil to biodiesel catalyzed by bronsted acid ionic liquid[J];Journal of University of Science and Technology Beijing;2011-11
5 ZHANG Zheng-jie,WANG Ping (School of Food,Beijing Technology and Business University,Beijing 100048,China);Optimization of Culture Conditions of Chlorella.sp[J];Journal of Beijing Technology and Business University(Natural Science Edition);2011-01
6 Huang Xianhong (Applied Chemistry, Hainan University, Haikou 571737, China);The Research Progress of Circulating Water Treatment Microbicides[J];Guangdong Chemical Industry;2009-09
7 LIU Zhi-hong1,DONG Bin2(1.Jiaxing Union Sewage Treatment Co.Ltd.,Jiaxing 314050,China;2.College of Environmental Science and Engineering,Tongji University,Shanghai 200092,China);Biofouling Control in Circulating Cooling Water System[J];China Water & Wastewater;2008-12
8 CHEN Jun-hui,WEI Dong(College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China);Bioenrichment of Fe,Zn and Ca by Heterotrophic Chlorella[J];Modern Food Science and Technology;2010-11
9 ZHAO Hua,DONG Xiao-yu,XIA Yuan-yuan,NIU Kun(Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin Industrial Microbiology Key Laboratory,College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China);Effects of Nitrogen Source on Photosynthesis and Pigment Accumulation of Chlorella sp.TCCC45058[J];Modern Food Science and Technology;2012-04
10 GE Zhen-zhen et al(The Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi,Jiangsu 214122);Effects of Fed-batch Cultivation and Nitrogen Deficiency on Lipid Yield of Chlorella vulgaris[J];Journal of Anhui Agricultural Sciences;2012-24
China Proceedings of conference Full-text Database 1 Hits
1 Liu Zhenqiang,Lu Xianghong~*,Yan Rongjun,Ao Hui,Mei Xiaodong,Gao Ting,Ji Jianbing (College of Chemical Engineering and Materials Science,Zhejiang University of Technology, Zhejiang Province Key Laboratory of biofuel,Hangzhou,310014,China);Advances on technology of high density and high lipid microalgae culture[A];Chinese Society of Agricultural Engineering[C];2011
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 Liu Xueming Liang Shizhong (The College of Food Engineering and Biotechnology of South China University of Technology Guangzhou);THE APPLICATION OF CHLORELLA IN FOOD INDUSTRY AND DEVELOPMENT OF CHLORELLA FOOD[J];Journal of Wuhan Food Industry College;1999-01
2 WU Qing-Yu and KUANG Mei (Department of Biology, Nanjing University, Nanjing 210008) Grant N. G. (The William Paterson College of New Jersey,Wayne, NJ 07470 U. S. A.);Growth, AEC and Pigments of Chlorella protothecoides in Two Strains Under the Autotrophic and Heterotrophic Conditions[J];;1992-03
3 LI ShiWeng(Department of Biology,Qingyang Teacher's College, XiFeng GanSu 745000)LI HuQian ZHANG JianJun(Shenzhen Yixing Photosynthesis Limited Company, Shenzhen 518008);PROGRESS IN STUDIES ON LARGESCALE CULTURE OF CHLORELLA[J];CHINESE BULLETIN OF BOTANY;1998-04
4 Liu XuemingLiang Shizhong (The College of Food Engineering and Biotechnology of South China University of Technology Guangzhou 510641);Advances in the Production of PUFAs by the Culture of Mocroalgae[J];CHINA OILS AND FATS;1999-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved